Sarhan H R, Williams L R, Foster H A
Department of Biological Sciences, University of Salford, UK.
J Dairy Res. 1991 Nov;58(4):477-83. doi: 10.1017/s0022029900030089.
A rapid fluorogenic medium was evaluated for the detection of Escherichia coli in dairy products. The medium was capable of detecting Esch. coli after 7.5 h incubation at 41.5 degrees C. Samples of pasteurized milk (136), raw milk (63), soft cheese (60) and pasteurized cream (39) were examined with media based on 4-methylumbelliferyl-beta-D-glucuronide (MUG-7) and Violet red bile agar and there were no significant differences between the numbers of Esch. coli detected on the two media. MUG-7 medium had a specificity of 98.6% and the small number of organisms giving a false positive reaction were identified as Klebsiella pneumoniae. The incidence of false negative results was approximately 2%. MUG-7 medium was suitable for pour plate, spread plate and membrane filtration methods. Possible applications of the method are discussed.
对一种快速荧光培养基进行了评估，用于检测乳制品中的大肠杆菌。该培养基在41.5摄氏度孵育7.5小时后能够检测出大肠杆菌。用基于4-甲基伞形酮-β-D-葡萄糖醛酸苷（MUG-7）的培养基和紫红胆盐琼脂对巴氏杀菌牛奶（136份）、生牛奶（63份）、软奶酪（60份）和巴氏杀菌奶油（39份）样本进行检测，两种培养基检测出的大肠杆菌数量之间没有显著差异。MUG-7培养基的特异性为98.6%，少数产生假阳性反应的微生物被鉴定为肺炎克雷伯菌。假阴性结果的发生率约为2%。MUG-7培养基适用于倾注平板法、涂布平板法和膜过滤法。讨论了该方法的可能应用。
