用于检测食品中大肠杆菌的荧光测定法评估。
Evaluation of a fluorogenic assay for detection of Escherichia coli in foods.
作者信息
Robison B J
出版信息
Appl Environ Microbiol. 1984 Aug;48(2):285-8. doi: 10.1128/aem.48.2.285-288.1984.
Abstract
A fluorogenic assay procedure with 4-methylumbelliferyl-beta-D-glucuronide incorporated into lauryl sulfate broth was evaluated to detect and confirm the presence of Escherichia coli in foods. Fluorescence is indicative of the presence of E. coli; extensive biochemical confirmation is unnecessary with this assay. The 4-methylumbelliferyl-beta-D-glucuronide assay was tested concurrently with our present methodology for detection of E. coli on 270 samples of raw ingredients and powdered food products. Total agreement between the two methods was 94.8%; there was a false-positive rate of 4.8% and no false-negatives. We found the 4-methylumbelliferyl-beta-D-glucuronide assay to be rapid, accurate, simple to perform, and inexpensive.
摘要
对一种将4-甲基伞形酮基-β-D-葡萄糖醛酸苷加入月桂基硫酸盐肉汤中的荧光测定程序进行了评估，以检测和确认食品中大肠杆菌的存在。荧光表明存在大肠杆菌；该测定无需广泛的生化确认。4-甲基伞形酮基-β-D-葡萄糖醛酸苷测定法与我们目前检测270份原料和粉状食品样本中大肠杆菌的方法同时进行了测试。两种方法的总一致性为94.8%；假阳性率为4.8%，无假阴性。我们发现4-甲基伞形酮基-β-D-葡萄糖醛酸苷测定法快速、准确、操作简单且成本低廉。
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