黑曲霉MIUG 16产生的果胶分解复合物中具有新生化特性的酶。

Enzymes with new biochemical properties in the pectinolytic complex produced by Aspergillus niger MIUG 16.

作者信息

Dinu Diana, Nechifor Marina Tamara, Stoian Gheorghe, Costache Marieta, Dinischiotu Anca

机构信息

Department of Biochemistry, Faculty of Biology, University of Bucharest, 91-95 Splaiul Independentei, Bucharest 050095, Romania.

出版信息

J Biotechnol. 2007 Aug 31;131(2):128-37. doi: 10.1016/j.jbiotec.2007.06.005. Epub 2007 Jun 23.

DOI:10.1016/j.jbiotec.2007.06.005

PMID:17655960

Abstract

A comprehensive study on the purification and characterization of pectinolytic enzymes produced by Aspergillus niger MIUG 16 on raw materials solid-state fermentation is reported. Five pectinolytic enzymes were purified using a combination of chromatographic techniques. The properties of these homogenous enzymes were analyzed. The purified enzymes were classified with respect to their biochemical properties and substrate specificity. Among these proteins, one revealed polygalacturonase activity, another appeared to be a pectin methylesterase and three were identified as pectate lyases. The capacity of the fungus A. niger to produce pectate lyases with optimum pH in acidic domain was reported for the first time.

摘要

报道了一项关于黑曲霉MIUG 16在原料固态发酵中产生的果胶分解酶的纯化和特性的综合研究。使用色谱技术组合纯化了五种果胶分解酶。分析了这些纯酶的性质。根据其生化性质和底物特异性对纯化的酶进行了分类。在这些蛋白质中，一种显示出聚半乳糖醛酸酶活性，另一种似乎是果胶甲基酯酶，三种被鉴定为果胶酸裂解酶。首次报道了黑曲霉在酸性域产生具有最佳pH值的果胶酸裂解酶的能力。