大型瘤胃密螺旋体的果胶分解酶
Pectinolytic enzymes of large rumen treponemes.
作者信息
Wojciechowicz M, Ziołecki A
出版信息
Appl Environ Microbiol. 1979 Jan;37(1):136-42. doi: 10.1128/aem.37.1.136-142.1979.
Abstract
Large spiral organisms isolated from the rumen of cattle produced and released into the external environment a complex of pectinolytic enzymes, consisting mainly of poly(1,4-alpha-D-galacturonide) lyase (EC 4.2.2.2, formerly EC 4.2.99.3), most active at pH 8.0 to 9.0, and another enzyme acting at pH below 7.0, probably a poly(1,4-alpha-D-galacturonide) glycanohydrolase (EC 3.2.1.15). The mixture of enzymes degraded polygalacturonate to saturated and unsaturated monogalacturonates as the end products. A pectin pectylhydrolase (pectinesterase) (EC 3.1.1.11) was also present in the clarified cultures.
摘要
从牛瘤胃中分离出的大型螺旋状微生物产生并释放到外部环境中的一组果胶分解酶，主要由聚（1,4-α-D-半乳糖醛酸）裂解酶（EC 4.2.2.2，原EC 4.2.99.3）组成，在pH 8.0至9.0时活性最高，还有另一种在pH低于7.0时起作用的酶，可能是聚（1,4-α-D-半乳糖醛酸）聚糖水解酶（EC 3.2.1.15）。该酶混合物将聚半乳糖醛酸降解为饱和和不饱和单半乳糖醛酸作为终产物。澄清培养物中还存在一种果胶果胶水解酶（果胶酯酶）（EC 3.1.1.11）。
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