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用于生物样本扫描电子显微镜检查的传统样本制备技术。

Conventional specimen preparation techniques for scanning electron microscopy of biological specimens.

作者信息

Bozzola John J

机构信息

IMAGE Center, Southern Illinois University, Carbondale, IL, USA.

出版信息

Methods Mol Biol. 2007;369:449-66. doi: 10.1007/978-1-59745-294-6_22.

Abstract

This chapter covers conventional methods for preparing biological specimens for examination in the scanning electron microscope (SEM). Techniques for handling cells grown in liquid culture, as well as on substrates such as culture dishes, slide culture chambers or agar, are discussed. These methods may be used to process most cultured organisms as well as whole botanical and zoological specimens.

摘要

本章介绍了用于制备生物样本以在扫描电子显微镜(SEM)下检查的传统方法。讨论了处理在液体培养中生长的细胞的技术,以及在诸如培养皿、玻片培养室或琼脂等基质上生长的细胞的处理技术。这些方法可用于处理大多数培养的生物体以及整个植物和动物标本。

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