用于培养细胞透射电子显微镜检查的传统标本制备技术。

Conventional specimen preparation techniques for transmission electron microscopy of cultured cells.

作者信息

Bozzola John J

机构信息

IMAGE Center, Southern Illinois University, Carbondale, IL, USA.

出版信息

Methods Mol Biol. 2007;369:1-18. doi: 10.1007/978-1-59745-294-6_1.

DOI:10.1007/978-1-59745-294-6_1

PMID:17656743

Abstract

This chapter covers the conventional methods and considerations for preparing cultured cells for examination in the transmission electron microscope. Techniques for handling cells grown in liquid culture, as well as on substrates such as culture dishes or agar, are discussed. Directions are given on how to prepare the most commonly used buffers, fixatives, enrobement media, and embedding resins. These methods may be applied to most cultured organisms, from bacteria to mammalian cells.

摘要

本章介绍了用于透射电子显微镜检查的培养细胞制备的传统方法及注意事项。讨论了处理液体培养以及在培养皿或琼脂等基质上生长的细胞的技术。给出了如何制备最常用的缓冲液、固定剂、包埋介质和包埋树脂的说明。这些方法可应用于从细菌到哺乳动物细胞的大多数培养生物体。