通过低温扫描电子显微镜观察木质组织中水分分布的低温刨平技术。

Cryoplaning technique for visualizing the distribution of water in woody tissues by cryoscanning electron microscopy.

作者信息

Utsumi Yasuhiro, Sano Yuzou

机构信息

Shiiba Research Forest, Graduate School of Agriculture, Kyushu Uiversity, Japan.

出版信息

Methods Mol Biol. 2007;369:497-506. doi: 10.1007/978-1-59745-294-6_24.

DOI:10.1007/978-1-59745-294-6_24

PMID:17656766

Abstract

The protocol of cryoplaning techniques that to examine the distribution of water in living tree stems by cryoscanning electron microscopy have been developed and described. In brief, the procedures are as follows: First, a portion of transpiring stem is frozen in the standing state with liquid nitrogen to stabilize the water that is present in the conducting tissue. After filling with liquid nitrogen, discs are then collected from the frozen portion of the stem and stored in liquid nitrogen. The surface of disc is cleanly cut using a sliding microtome in a low temperature room at -20 degrees C. Finally, the frozen sample is examined in a cryoscanning electron microscope after freeze-etching and metal coating.

摘要

已经开发并描述了通过低温扫描电子显微镜检查活树干中水分分布的低温切片技术方案。简而言之，步骤如下：首先，将一部分正在蒸腾的茎干在液氮中以直立状态冷冻，以稳定存在于输导组织中的水分。充满液氮后，从茎干的冷冻部分收集切片并储存在液氮中。在-20℃的低温室中使用滑动切片机将切片表面干净地切割。最后，在冷冻蚀刻和金属镀膜后，在低温扫描电子显微镜下检查冷冻样品。