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水稻第8染色体上孕穗期耐冷性的一个数量性状位点。

A quantitative trait locus for cold tolerance at the booting stage on rice chromosome 8.

作者信息

Kuroki Makoto, Saito Koji, Matsuba Shuichi, Yokogami Narifumi, Shimizu Hiroyuki, Ando Ikuo, Sato Yutaka

机构信息

National Agricultural Research Center for Hokkaido Region, National Agriculture and Food Research Organization (NARO), 1 Hitsujigaoka, Toyohira, Sapporo, Hokkaido 062-8555, Japan.

出版信息

Theor Appl Genet. 2007 Sep;115(5):593-600. doi: 10.1007/s00122-007-0589-y. Epub 2007 Jul 27.

Abstract

A quantitative trait locus (QTL) for cold tolerance at the booting stage of a cold-tolerant rice breeding line, Hokkai-PL9, was analyzed. A total of 487 simple sequence repeat (SSR) markers distributed throughout the genome were used to survey for polymorphism between Hokkai-PL9 and a cold-sensitive breeding line, Hokkai287, and 54 markers were polymorphic. Single marker analysis revealed that markers on chromosome 8 are associated with cold tolerance. By interval mapping using an F(2) population between Hokkai-PL9 and Hokkai287, a QTL for cold tolerance was detected on the short arm of chromosome 8. The QTL explains 26.6% of the phenotypic variance, and its additive effect is 11.4%. Substitution mapping suggested that the QTL is located in a 193-kb interval between SSR markers RM5647 and PLA61. We tentatively designated the QTL as qCTB8 (quantitative trait locus for cold tolerance at the booting stage on chromosome 8).

摘要

对耐寒水稻育种系北海PL9孕穗期耐寒性的数量性状位点(QTL)进行了分析。使用分布于全基因组的487个简单序列重复(SSR)标记来检测北海PL9与冷敏感育种系北海287之间的多态性,其中54个标记具有多态性。单标记分析表明,第8号染色体上的标记与耐寒性相关。通过利用北海PL9和北海287之间的F(2)群体进行区间作图,在第8号染色体短臂上检测到一个耐寒QTL。该QTL解释了26.6%的表型变异,其加性效应为11.4%。代换作图表明,该QTL位于SSR标记RM5647和PLA61之间193 kb的区间内。我们暂时将该QTL命名为qCTB8(第8号染色体孕穗期耐寒性数量性状位点)。

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