Kuroki Makoto, Saito Koji, Matsuba Shuichi, Yokogami Narifumi, Shimizu Hiroyuki, Ando Ikuo, Sato Yutaka
National Agricultural Research Center for Hokkaido Region, National Agriculture and Food Research Organization (NARO), 1 Hitsujigaoka, Toyohira, Sapporo, Hokkaido 062-8555, Japan.
Theor Appl Genet. 2007 Sep;115(5):593-600. doi: 10.1007/s00122-007-0589-y. Epub 2007 Jul 27.
A quantitative trait locus (QTL) for cold tolerance at the booting stage of a cold-tolerant rice breeding line, Hokkai-PL9, was analyzed. A total of 487 simple sequence repeat (SSR) markers distributed throughout the genome were used to survey for polymorphism between Hokkai-PL9 and a cold-sensitive breeding line, Hokkai287, and 54 markers were polymorphic. Single marker analysis revealed that markers on chromosome 8 are associated with cold tolerance. By interval mapping using an F(2) population between Hokkai-PL9 and Hokkai287, a QTL for cold tolerance was detected on the short arm of chromosome 8. The QTL explains 26.6% of the phenotypic variance, and its additive effect is 11.4%. Substitution mapping suggested that the QTL is located in a 193-kb interval between SSR markers RM5647 and PLA61. We tentatively designated the QTL as qCTB8 (quantitative trait locus for cold tolerance at the booting stage on chromosome 8).
对耐寒水稻育种系北海PL9孕穗期耐寒性的数量性状位点(QTL)进行了分析。使用分布于全基因组的487个简单序列重复(SSR)标记来检测北海PL9与冷敏感育种系北海287之间的多态性,其中54个标记具有多态性。单标记分析表明,第8号染色体上的标记与耐寒性相关。通过利用北海PL9和北海287之间的F(2)群体进行区间作图,在第8号染色体短臂上检测到一个耐寒QTL。该QTL解释了26.6%的表型变异,其加性效应为11.4%。代换作图表明,该QTL位于SSR标记RM5647和PLA61之间193 kb的区间内。我们暂时将该QTL命名为qCTB8(第8号染色体孕穗期耐寒性数量性状位点)。