Kudo H, Uyeda I, Shikata E
Department of Botany, Faculty of Agriculture, Hokkaido University, Sapporo, Japan.
J Gen Virol. 1991 Dec;72 ( Pt 12):2857-66. doi: 10.1099/0022-1317-72-12-2857.
The 5'- and 3'-terminal nucleotide sequences of the dsRNA genome segments of rice dwarf virus (RDV) and rice gall dwarf virus (RGDV), members of the Phytoreovirus genus of the Reoviridae family, were determined and compared with those of wound tumour virus (WTV). The 3' tetranucleotides of the plus strand of all genome segments of RDV and RGDV were found to be the same (---UGAU 3'), except for segment 9 of RDV which had the 3'-terminal sequence---CGAU 3'. The conserved 3'-terminal sequence (---UGAU 3') was the same as that found in the genome segments of WTV, another member of the Phytoreovirus genus. On the other hand, the 5' termini of the plus strands of RDV and RGDV were found to have two or three types of common sequence. RDV had either 5' GGCAAA--- or 5' GGUAAA---, whereas RGDV had 5' GGCAUUUU---, 5' GGUAUUUU--- or 5' GGUAAUUU---. These conserved sequences were similar to the conserved 5'-terminal sequence of WTV (5' GGUAUU---). Although the three viruses differ in plant host range, tissue specificity, vector specificity and disease symptom expression, these results suggest that they have a common ancestral origin.
测定了呼肠孤病毒科植物呼肠孤病毒属成员水稻矮缩病毒（RDV）和水稻瘤矮病毒（RGDV）双链RNA基因组片段的5'和3'末端核苷酸序列，并与创伤肿瘤病毒（WTV）的相应序列进行了比较。发现RDV和RGDV所有基因组片段正链的3'四核苷酸相同（---UGAU 3'），但RDV的片段9具有3'末端序列---CGAU 3'。保守的3'末端序列（---UGAU 3'）与植物呼肠孤病毒属的另一个成员WTV基因组片段中的序列相同。另一方面，发现RDV和RGDV正链的5'末端有两种或三种常见序列。RDV的5'末端为GGCAAA--- 或GGUAAA---，而RGDV的5'末端为GGCAUUUU---、GGUAUUUU--- 或GGUAAUUU---。这些保守序列与WTV的保守5'末端序列（5' GGUAUU---）相似。尽管这三种病毒在植物宿主范围、组织特异性、载体特异性和疾病症状表现方面存在差异，但这些结果表明它们有共同的祖先起源。
