水稻矮缩病毒基因组S10片段的核苷酸序列。

Nucleotide sequence of segment S10 of the rice dwarf virus genome.

作者信息

Omura T, Minobe Y, Tsuchizaki T

机构信息

National Agriculture Research Center, Ibaraki, Japan.

出版信息

J Gen Virol. 1988 Jan;69 ( Pt 1):227-31. doi: 10.1099/0022-1317-69-1-227.

DOI:10.1099/0022-1317-69-1-227

PMID:3335833

Abstract

DNA complementary to the tenth largest (S10) of the 12 genome segments of rice dwarf virus (RDV) was cloned and its sequence was determined. This genome segment is 1319 nucleotides in length and has a single long open reading frame extending for 1059 nucleotides from the first AUG triplet (residues 27 to 29). The predicted translation product comprises 352 amino acids and has a mol. wt. of 39094. RDV transcripts synthesized in vitro have the same polarity as the plus strand of the genome. Terminal sequences were (+) 5' GGUA---UGAU 3' and (-) 3' CCAU---ACUA 5' which are similar to those of wound tumour virus RNA.

摘要

克隆了与水稻矮缩病毒（RDV）12个基因组片段中第十大（S10）片段互补的DNA，并测定了其序列。该基因组片段长度为1319个核苷酸，有一个单一的长开放阅读框，从第一个AUG三联体（第27至29位残基）开始延伸1059个核苷酸。预测的翻译产物包含352个氨基酸，分子量为39094。体外合成的RDV转录本与基因组正链具有相同的极性。末端序列为（+）5' GGUA---UGAU 3' 和（-）3' CCAU---ACUA 5'，与伤口肿瘤病毒RNA的末端序列相似。