优化多色免疫表型分析检测法。
Optimizing a multicolor immunophenotyping assay.
作者信息
Mahnke Yolanda D, Roederer Mario
机构信息
ImmunoTechnology Section, Vaccine Research Center, NIAID, NIH, 40 Convent Drive, Room 5509, Bethesda, MD 20892, USA.
出版信息
Clin Lab Med. 2007 Sep;27(3):469-85, v. doi: 10.1016/j.cll.2007.05.002.
Abstract
Flow cytometry-based immunophenotyping assays have become increasingly multiparametric, concomitantly analyzing multiple cellular parameters. To maximize the quality of the information obtained, antibody conjugate panels need to be developed with care, including requisite controls at every step. Such an optimization procedure for multicolor immunophenotyping assays is time consuming, but the value of having a reliable antibody conjugate panel that provides for sensitive detection of all molecules of interest justifies this time investment. This article outlines important considerations and procedures to undertake for the successful design and development of multicolor flow cytometry panels.
摘要
基于流式细胞术的免疫表型分析检测已变得越来越具有多参数性，可同时分析多个细胞参数。为了最大化所获信息的质量，需要精心开发抗体偶联物面板，包括在每个步骤设置必要的对照。这种用于多色免疫表型分析检测的优化程序很耗时，但拥有一个能灵敏检测所有感兴趣分子的可靠抗体偶联物面板的价值证明了这种时间投入是值得的。本文概述了成功设计和开发多色流式细胞术面板需考虑的重要因素和步骤。
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