研究人类免疫组：全面白细胞免疫表型分析的复杂性
Studying the human immunome: the complexity of comprehensive leukocyte immunophenotyping.
作者信息
Biancotto Angélique, McCoy J Philip
机构信息
Center for Human Immunology, Autoimmunity, and Inflammation, National Institutes of Health, Bethesda, MD, 20892, USA.
出版信息
Curr Top Microbiol Immunol. 2014;377:23-60. doi: 10.1007/82_2013_336.
Abstract
A comprehensive study of the cellular components of the immune system requires both deep and broad immunophenotyping of numerous cell populations in an efficient and practical manner. In this chapter, we describe the technical aspects of studying the human immunome using high-dimensional (15 color) fluorescence-based immunophenotyping. We focus on the technical aspects of polychromatic flow cytometry and the initial stages in developing a panel for comprehensive leukocyte immunophenotyping (CLIP). We also briefly discuss how this panel is being used and the challenges of encyclopedic analysis of these rich data sets.
摘要
对免疫系统的细胞成分进行全面研究，需要以高效且实用的方式对众多细胞群体进行深入且广泛的免疫表型分析。在本章中，我们描述了使用基于高维（15色）荧光的免疫表型分析来研究人类免疫组的技术方面。我们重点关注多色流式细胞术的技术方面以及开发用于全面白细胞免疫表型分析（CLIP）的检测板的初始阶段。我们还简要讨论了该检测板的使用方式以及对这些丰富数据集进行百科全书式分析所面临的挑战。
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