Oldaker Teri A
Genzyme Genetics, 5300 McConnell Avenue, Los Angeles, CA 90066, USA.
Clin Lab Med. 2007 Sep;27(3):671-85, viii. doi: 10.1016/j.cll.2007.05.009.
Immunophenotyping by flow cytometry is a robust and highly complex technology used in the enumeration and characterization of leukocytes, including normal lymphocyte subsets and hematologic neoplasms. Samples consist of peripheral blood and many times irreplaceable samples, such as bone marrow and fresh tissue. These samples are collected, transported, prepared, analyzed, and interpreted, resulting in diagnostic and prognostic information. Such information is critical to treatment decisions for patients. In order to obtain accurate and reproducible results, it is essential to have optimized and standardized procedures, rigorous quality control, and assurance programs encompassing preanalytic, analytic, and postanalytic processes.
流式细胞术免疫表型分析是一种强大且高度复杂的技术,用于白细胞的计数和特征分析,包括正常淋巴细胞亚群和血液系统肿瘤。样本包括外周血以及很多时候不可替代的样本,如骨髓和新鲜组织。这些样本要经过采集、运输、制备、分析和解读,从而得出诊断和预后信息。此类信息对于患者的治疗决策至关重要。为了获得准确且可重复的结果,必须要有经过优化和标准化的程序、涵盖分析前、分析中和分析后过程的严格质量控制和保证计划。