Greig Bruce
Hematopathology/Flow Cytometry, Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN, USA.
Methods Mol Biol. 2019;2032:227-279. doi: 10.1007/978-1-4939-9650-6_14.
Applications of immunophenotyping using flow cytometry offer precise and accurate means for providing information used to both diagnose and monitor disease; they serve as a standard platform for many research endeavors that study discrete populations of biological entities. The proper use of this highly sophisticated technology requires daily and ongoing monitoring of both the instrument and the methodology. Best practices for this begin with quality control (QC) procedures designed to set up and monitor the instrument performance, the reagents, and the results to ensure that they are working properly both on the day of use and over time. If the results of those QC procedures are outside of acceptable then recording the corrective action taken must also be included in the quality control records. Quality assurance (QA) is a way to know that the three phases of testing, namely, preanalytic, analytic, and postanalytic procedures, are being followed. This chapter describes the procedures used to assess quality control as it pertains to flow cytometry and immunophenotyping in all three phases of testing.
使用流式细胞术进行免疫表型分析的应用为提供用于疾病诊断和监测的信息提供了精确且准确的手段；它们是许多研究离散生物实体群体的研究工作的标准平台。正确使用这项高度复杂的技术需要对仪器和方法进行日常和持续的监测。为此，最佳实践始于质量控制（QC）程序，该程序旨在设置和监测仪器性能、试剂和结果，以确保它们在使用当天以及随时间推移都能正常工作。如果这些QC程序的结果超出可接受范围，那么在质量控制记录中还必须包括所采取的纠正措施。质量保证（QA）是一种了解测试的三个阶段（即分析前、分析和分析后程序）是否得到遵循的方法。本章描述了在测试的所有三个阶段中用于评估与流式细胞术和免疫表型分析相关的质量控制的程序。
