曲古抑菌素A对胃癌细胞系SGC-7901的影响

[Impact of trichostatin A on gastric carcinoma cell line SGC-7901].

作者信息

Li Yun-long, Zou Xiao-ming, Guo Bao-liang, Li Xiao-lin, Yan Chao-qi, You Li-guang, Fu Song-bin

机构信息

Department of General Surgery, The Second Affiliated Hospital, Harbin Medical University, Harbin 150086, China.

出版信息

Zhonghua Wei Chang Wai Ke Za Zhi. 2007 Jul;10(4):376-9.

PMID:17659468

Abstract

OBJECTIVE

To investigate the effect of trichostatin A(TSA) on SGC- 7901 cells.

METHODS

Cytotoxicity and cell viability of gastric cancer cell line SGC- 7901 were assayed by MTT method. Morphologic assessment of apoptosis was performed with fluorescence microscope. Cell cycle and apoptosis rate were analyzed by flow cytometry. Histone H3 acetylation was detected by Western blot.

RESULTS

TSA showed apparently cytotoxicity in SGC- 7901 cells. The growth curve showed the growth ratio decreased with the increase of TSA concentration. Apoptosis rate were significantly different between TSA treated group(75 ng/ml for 72 h)and control group (P < 0.05). Morphologic changes of apoptosis including nuclear chromatin condensation and fluorescence strength were observed with fluorescence microscope.TSA treatment (75 ng/ml for 72 h) sensitively induced apoptosis in the cell,which was demonstrated by the migration of many cells to the sub- G1 phase,the reduction of G1- phase cells and the increment of apoptosis rate (29.54%) in flow cytometric analysis. The expression of acetylated histone H3 was increased in TSA group(75 ng/ml) for 48 h compared with control group by Western blot.

CONCLUSIONS

TSA can induce SGC- 7901 cell apoptosis. The expression of acetylated histone H3 may contribute to the apoptosis.

摘要

目的

研究曲古抑菌素A（TSA）对SGC - 7901细胞的作用。

方法

采用MTT法检测胃癌细胞系SGC - 7901的细胞毒性和细胞活力。用荧光显微镜进行凋亡的形态学评估。通过流式细胞术分析细胞周期和凋亡率。采用蛋白质免疫印迹法检测组蛋白H3的乙酰化。

结果

TSA对SGC - 7901细胞显示出明显的细胞毒性。生长曲线显示随着TSA浓度的增加，生长率下降。TSA处理组（75 ng/ml，作用72小时）与对照组的凋亡率有显著差异（P < 0.05）。用荧光显微镜观察到凋亡的形态学变化，包括核染色质浓缩和荧光强度。TSA处理（75 ng/ml，作用72小时）可诱导细胞发生凋亡，流式细胞术分析显示许多细胞迁移至亚G1期，G1期细胞减少，凋亡率增加（29.54%）。蛋白质免疫印迹法显示，与对照组相比，TSA组（75 ng/ml）作用48小时后乙酰化组蛋白H3的表达增加。