Zou Xiao-Ming, Li Yun-Long, Wang Hao, Cui Wu, Li Xiao-Lin, Fu Song-Bin, Jiang Hong-Chi
Department of General Surgery, the Second Clinical College, Harbin Medical University, Harbin 150086, Heilongjiang Province, China.
World J Gastroenterol. 2008 Aug 14;14(30):4810-5. doi: 10.3748/wjg.14.4810.
To explore the effect of trichostatin A (TSA) on apoptosis and acetylated histone H3 levels in gastric cancer cell lines BGC-823 and SGC-7901.
The effect of TSA on growth inhibition and apoptosis was examined by MTT, fluorescence microscopy and PI single-labeled flow cytometry. The acetylated histone H3 level was detected by Western blot.
TSA induced apoptosis in gastric cancer cell lines BGC-823 and SGC-7901 was in a dose and time-dependent manner. Apoptotic cells varied significantly between TSA treated groups (37.5 ng/mL 72 h for BGC-823 cell line and 75 ng/mL 72 h for SGC-7901 cell line) and control group (0.85+/-0.14 vs 1.14+/-0.07, P=0.02; 0.94+/-0.07 vs 1.15+/-0.06, P=0.02). Morphologic changes of apoptosis, including nuclear chromatin condensation and fluorescence strength, were observed under fluorescence microscopy. TSA treatment in BGC-823 and SGC-7901 cell lines obviously induced cell apoptosis, which was demonstrated by the increased percentage of sub-G1 phase cells, the reduction of G1-phase cells and the increase of apoptosis rates in flow cytometric analysis. The result of Western blot showed that the expression of acetylated histone H3 increased in BGC-823 and SGC-7901 TSA treatment groups as compared with the control group.
TSA can induce cell apoptosis in BGC-823 and SGC-7901 cell lines. The expression of acetylated histone H3 might be correlated with apoptosis.
探讨曲古抑菌素A（TSA）对胃癌细胞系BGC - 823和SGC - 7901凋亡及组蛋白H3乙酰化水平的影响。
采用MTT法、荧光显微镜观察及PI单标记流式细胞术检测TSA对细胞生长抑制及凋亡的影响；采用蛋白质免疫印迹法检测组蛋白H3乙酰化水平。
TSA诱导胃癌细胞系BGC - 823和SGC - 7901凋亡呈剂量和时间依赖性。TSA处理组（BGC - 823细胞系为37.5 ng/mL 72 h，SGC - 7901细胞系为75 ng/mL 72 h）与对照组（0.85±0.14比1.14±0.07，P = 0.02；0.94±0.07比1.15±0.06，P = 0.02）凋亡细胞差异显著；荧光显微镜下观察到凋亡的形态学变化，包括核染色质凝聚和荧光强度；流式细胞术分析显示，TSA处理BGC - 823和SGC - 7901细胞系明显诱导细胞凋亡，表现为亚G1期细胞百分比增加、G1期细胞减少及凋亡率升高；蛋白质免疫印迹结果显示，与对照组相比，TSA处理的BGC - 823和SGC - 7901组中组蛋白H3乙酰化表达增加。
TSA可诱导BGC - 823和SGC - 7901细胞系凋亡，组蛋白H3乙酰化表达可能与凋亡相关。
