Fukunaga Takashi, Soejima Hirofumi, Irie Atsushi, Sugamura Koichi, Oe Yoko, Tanaka Tomoko, Nagayoshi Yasuhiro, Kaikita Koichi, Sugiyama Seigo, Yoshimura Michihiro, Nishimura Yasuharu, Ogawa Hisao
Department of Cardiovascular Medicine, Graduate School of Medical Sciences, Kumamoto University, Kumamoto, Japan.
Am J Cardiol. 2007 Aug 1;100(3):483-8. doi: 10.1016/j.amjcard.2007.03.052. Epub 2007 Jun 13.
The percentage of CD4(+) T cells in blood is correlated with left ventricular dysfunction and decreased ejection fraction in heart disease. The aim of this study was to determine the relation between activation of CD4(+) T cells and New York Heart Association functional classes in chronic heart failure (HF) and differences in inflammatory activation between ischemic cardiomyopathy (IC) and idiopathic dilated cardiomyopathy (IDC). Blood samples were obtained from 47 patients with HF and 20 controls. Percentages of interferon-gamma-positive CD4(+) T cells (representative type 1 T-helper cells) and interleukin-4-positive CD4(+) T cells (representative type 2 T-helper cells) were analyzed using 3-color flow cytometry. The proportion of interferon-gamma-positive CD4(+) T cells was higher in patients with HF (28.96 +/- 12.90%) than in controls (18.12 +/- 5.28, p = 0.0006), but there was no difference in percentage of interleukin-4-positive CD4(+) T cells between the 2 groups. The proportion of interferon-gamma-positive CD4(+) T cells and plasma B-type natriuretic peptide levels increased with worsening of New York Heart Association functional class in the IC and IDC groups. The proportion of interferon-gamma-positive CD4(+) T cells in the IC group (33.88 +/- 13.33%) was higher than in the IDC group (22.33 +/- 8.88%, p = 0.002); however, plasma B-type natriuretic peptide levels were higher in the IDC group (358.0 pg/ml, 327.5 to 1,325.7) than in the IC group (82.7 pg/ml, 34.7 to 252.9, p = 0.019). In conclusion, we demonstrated pronounced type 1 T-helper cell activation in patients with HF in proportion to severity of HF and that the specificity of T-cell activation differs between patients with IC and those with IDC.
血液中CD4(+) T细胞的百分比与心脏病患者的左心室功能障碍及射血分数降低相关。本研究的目的是确定慢性心力衰竭（HF）患者中CD4(+) T细胞激活与纽约心脏协会功能分级之间的关系，以及缺血性心肌病（IC）和特发性扩张型心肌病（IDC）之间炎症激活的差异。从47例HF患者和20例对照者中采集血样。使用三色流式细胞术分析干扰素-γ阳性CD4(+) T细胞（代表性的1型辅助性T细胞）和白细胞介素-4阳性CD4(+) T细胞（代表性的2型辅助性T细胞）的百分比。HF患者中干扰素-γ阳性CD4(+) T细胞的比例（28.96±12.90%）高于对照者（18.12±5.28，p = 0.0006），但两组间白细胞介素-4阳性CD4(+) T细胞的百分比无差异。IC组和IDC组中，随着纽约心脏协会功能分级的恶化，干扰素-γ阳性CD4(+) T细胞的比例和血浆B型利钠肽水平升高。IC组中干扰素-γ阳性CD4(+) T细胞的比例（33.88±13.33%）高于IDC组（22.33±8.88%，p = 0.002）；然而，IDC组的血浆B型利钠肽水平（358.0 pg/ml，327.5至1325.7）高于IC组（82.7 pg/ml，34.7至252.9，p = 0.019）。总之，我们证明HF患者中存在明显的1型辅助性T细胞激活，其与HF严重程度成比例，且IC患者和IDC患者的T细胞激活特异性不同。
