使用多间隔区分型在种水平上鉴定立克次氏体分离株。
Identification of rickettsial isolates at the species level using multi-spacer typing.
作者信息
Fournier Pierre-Edouard, Raoult Didier
机构信息
Unité des rickettsies, IFR 48, CNRS UMR 6020, Faculté de médecine, Université de la Méditerranée, Marseille cedex 05, France.
出版信息
BMC Microbiol. 2007 Jul 30;7:72. doi: 10.1186/1471-2180-7-72.
BACKGROUND
In order to estimate whether multi-spacer typing (MST), based on the sequencing of variable intergenic spacers, could serve for the identification of Rickettsia at the species level, we applied it to 108 rickettsial isolates or arthropod amplicons that include representatives of 23 valid Rickettsia species.
RESULTS
MST combining the dksA-xerC, mppA-purC, and rpmE-tRNAfMet spacer sequences identified 61 genotypes, allowing the differentiation of each species by at least one distinct genotype. In addition, MST was discriminatory at the strain level in six species for which several isolates or arthropod amplicons were available.
CONCLUSION
MST proved to be a reproducible and high-resolution genotyping method allowing clear identification of rickettsial isolates at the species level and further additional differentiation of strains within some species.
背景
为了评估基于可变基因间隔区测序的多间隔区分型（MST）是否可用于立克次体属物种水平的鉴定，我们将其应用于108株立克次体分离株或节肢动物扩增子，其中包括23个有效立克次体物种的代表。
结果
结合dksA-xerC、mppA-purC和rpmE-tRNAfMet间隔区序列的MST鉴定出61种基因型，使得每个物种至少可通过一种独特的基因型进行区分。此外，对于有多个分离株或节肢动物扩增子的六个物种，MST在菌株水平上具有鉴别能力。
结论
MST被证明是一种可重复且高分辨率的基因分型方法，能够在物种水平上清晰鉴定立克次体分离株，并在某些物种内进一步区分菌株。
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