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新型实时 PCR 技术可区分 spp. 和.

New Real-Time PCRs to Differentiate spp. and .

机构信息

Istituto Zooprofilattico Sperimentale della Sicilia, Via Gino Marinuzzi 3, 90100 Palermo, Italy.

Department of Chemical, Biological, Pharmaceutical and Environmental Science, Università degli Studi di Messina, 98122 Messina, Italy.

出版信息

Molecules. 2020 Sep 27;25(19):4431. doi: 10.3390/molecules25194431.

DOI:10.3390/molecules25194431
PMID:32992475
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7582818/
Abstract

species are an important cause of emerging infectious diseases in people and animals, and rickettsiosis is one of the oldest known vector-borne diseases. Laboratory diagnosis of is complex and time-consuming. This study was aimed at developing two quantitative real-time PCRs targeting and genes for the detection, respectively, of spp. and DNA. Primers were designed following an analysis of gene sequences. The assays were optimized using SYBR Green and TaqMan methods and tested for sensitivity and specificity. This study allowed the development of powerful diagnostic methods, able to detect and quantify spp. DNA and differentiate species.

摘要

物种是导致人类和动物新发传染病的一个重要原因，而立克次体病是已知最古老的虫媒传染病之一。的实验室诊断复杂且耗时。本研究旨在开发两种针对和基因的定量实时 PCR，分别用于检测 spp. 和 DNA。根据基因序列分析设计了引物。使用 SYBR Green 和 TaqMan 方法对检测进行了优化，并测试了其灵敏度和特异性。本研究开发了强大的诊断方法，能够检测和定量 spp. DNA 并区分物种。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0484/7582818/316834429a1d/molecules-25-04431-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0484/7582818/7e6839ed4543/molecules-25-04431-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0484/7582818/9019ad953eb9/molecules-25-04431-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0484/7582818/5ec9d6cab1d4/molecules-25-04431-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0484/7582818/316834429a1d/molecules-25-04431-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0484/7582818/7e6839ed4543/molecules-25-04431-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0484/7582818/9019ad953eb9/molecules-25-04431-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0484/7582818/5ec9d6cab1d4/molecules-25-04431-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0484/7582818/316834429a1d/molecules-25-04431-g004.jpg

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