Parnes Doris, Jacoby Vered, Sharabi Avital, Schlesinger Hadassa, Brand Thomas, Kessler-Icekson Gania
Basil and Gerald Felsenstein Medical Research Center, Sackler Faculty of Medicine, Tel-Aviv University, Beilinson Campus, Rabin Medical Center, Petach-Tikva 49100, Israel.
Biochim Biophys Acta. 2007 Sep-Oct;1769(9-10):586-92. doi: 10.1016/j.bbaexp.2007.06.001. Epub 2007 Jun 16.
Three Popeye domain-containing (Popdc 1-3) family-members are known in vertebrates. Their exact function is as yet unknown although involvement in cell adhesion has been suggested. We report herein sequencing of the rat Popdc 1-3 cDNAs that show high homology to other vertebrate orthologs and are expressed primarily in the heart and skeletal muscles. Popdc2 splice variants were identified, with Popdc2C showing a distinctive age-dependent decline. In isolated cardiomyocytes, Popdc genes were negatively regulated by serum, an effect that was reversed by EGFR-kinase inhibition, suggesting an EGFR-dependent modulation of Popdc gene expression.
在脊椎动物中已知有三种含波佩域（Popdc 1 - 3）的家族成员。尽管有人提出它们参与细胞黏附，但其确切功能尚不清楚。我们在此报告大鼠Popdc 1 - 3 cDNA的测序结果，这些cDNA与其他脊椎动物的直系同源物具有高度同源性，并且主要在心脏和骨骼肌中表达。鉴定出了Popdc2剪接变体，其中Popdc2C显示出明显的年龄依赖性下降。在分离的心肌细胞中，Popdc基因受到血清的负调控，而表皮生长因子受体（EGFR）激酶抑制可逆转这种效应，这表明Popdc基因表达存在EGFR依赖性调节。
