Pirenne J, D'Silva M, Nakhleh R E, Lee S, Dunn D L
Department of Surgery, University of Minnesota, Minneapolis.
Microsurgery. 1991;12(6):378-84. doi: 10.1002/micr.1920120603.
Although transplantation of multiple abdominal viscera (MOTx) has been performed in humans, reproducible animal models and extensive laboratory work are needed to explore the physiological and immunological aspects of this new transplant procedure. We therefore developed a microsurgical model that allows en bloc transplantation of three major abdominal viscera. Using isogeneic rat strain combinations, 30% of the liver, pancreas, and duodenum and a segment of small bowel (SB) were heterotopically transplanted. A segment of aorta that included the celiac axis and the superior mesenteric artery provided the blood supply to the graft. Venous outflow occurred via the graft suprahepatic vena cava into the recipient's infrahepatic vena cava. The graft SB was anastomosed to the native SB. After a training period (N = 15), we could achieve a success rate of 83% (N = 23). Mean operation time was 105 minutes. Macroscopic and microscopic appearance of transplanted organs was normal. This study documents the feasibility of MOTx in the rat and provides a microsurgical model that should facilitate preclinical experimental research in this area.
尽管人类已经开展了多脏器移植（MOTx），但仍需要可重复的动物模型和大量的实验室工作来探索这种新移植手术的生理和免疫方面。因此，我们开发了一种显微手术模型，可实现三个主要腹部脏器的整块移植。使用同基因大鼠品系组合，将肝脏、胰腺和十二指肠的30%以及一段小肠（SB）进行异位移植。一段包含腹腔干和肠系膜上动脉的主动脉为移植物提供血液供应。静脉血通过移植物肝上腔静脉流入受体的肝下腔静脉。将移植物小肠与受体的原生小肠进行吻合。经过一段训练期（N = 15）后，我们的成功率达到了83%（N = 23）。平均手术时间为105分钟。移植器官的宏观和微观外观均正常。本研究证明了大鼠多脏器移植的可行性，并提供了一种显微手术模型，应有助于该领域的临床前实验研究。
