Pirenne J, Nakhleh R E, Dunn D L
Department of Surgery, University of Minnesota, Minneapolis 55455.
J Surg Res. 1991 Jun;50(6):622-8. doi: 10.1016/0022-4804(91)90052-n.
A rat model of multiorgan transplantation (MOTx) including the liver, pancreas, duodenum, and variable segments of small bowel (SB) was used to ascertain whether graft-versus-host disease (GVHD) could be produced by this procedure. Rats receiving an isogeneic multiorgan transplant (MOT) survived more than 150 days. MOTx was then performed in the Lewis to (Lewis x Brown Norway) LBNF1 semiallogeneic combination, ensuring unidirectional GVHD. En bloc transplantation of the liver, pancreas, duodenum, and entire jejunum provoked lethal GVHD in all animals, and the mean time to recipient death (MT) was 16.5 +/- 0.43. When only one-half of the jejunum was included in the MOT, lethal GVHD similarly occurred in 100% of animals and the MT was 18 +/- 0.86. Finally, when only liver, pancreas, and duodenum were transplanted, the incidence of lethal GVHD was reduced to 50% (P less than 0.1). In those rats that died of GVHD, MT was 16 +/- 0.33. Fifty percent of the rats in this group, however, recovered from a milder form of GVHD and survived more than 150 days. These results demonstrate that MOTx induces GVHD and that the lethality of this process correlates with the inclusion of the SB in the graft and thus, with the overall amount of transplanted lymphoid tissue.
使用包括肝脏、胰腺、十二指肠和小肠（SB）不同节段的多器官移植（MOTx）大鼠模型来确定该手术是否会引发移植物抗宿主病（GVHD）。接受同基因多器官移植（MOT）的大鼠存活超过150天。然后在Lewis与（Lewis×Brown Norway）LBNF1半同种异体组合中进行MOTx，确保单向GVHD。肝脏、胰腺、十二指肠和整个空肠的整块移植在所有动物中引发了致命的GVHD，受体死亡的平均时间（MT）为16.5±0.43天。当MOT中仅包含一半空肠时，100%的动物同样发生了致命的GVHD，MT为18±0.86天。最后，当仅移植肝脏、胰腺和十二指肠时，致命GVHD的发生率降至50%（P<0.1）。在那些死于GVHD的大鼠中，MT为16±0.33天。然而，该组中有50%的大鼠从较轻形式的GVHD中恢复过来，并存活超过150天。这些结果表明，MOTx会诱发GVHD，并且这一过程的致死性与移植物中包含SB相关，因此与移植的淋巴组织总量相关。
