用于基因递送的以钌（II）三联吡啶为中心的聚乙二胺
Ruthenium(II) tris(bipyridine)-centered poly(ethylenimine) for gene delivery.
作者信息
Fiore Gina L, Edwards James M, Payne Sarah J, Klinkenberg Jessica L, Gioeli Daniel G, Demas J N, Fraser Cassandra L
机构信息
Department of Chemistry, University of Virginia, McCormick Road, P.O. Box 400319, Charlottesville, Virginia 22904, USA.
出版信息
Biomacromolecules. 2007 Sep;8(9):2829-35. doi: 10.1021/bm700481h. Epub 2007 Jul 31.
Ruthenium(II) tris(bipyridine)-centered poly(ethylenimine) (Ru PEI) was synthesized via acid hydrolysis of Ru tris(bipyridine)-centered poly(2-ethyl-2-oxazoline) (Ru PEOX), and the luminescence, DNA entrapment, and transfection efficiencies were evaluated. Emission maxima for Ru PEI samples are red-shifted compared to Ru PEOX precursors, and the luminescence lifetimes are shorter in both methanol and aqueous solutions. Slower oxygen quenching of Ru PEOX and Ru PEI luminescence versus [Ru(bpy)3]Cl2 (bpy = bipyridine) is attributed to polymer shielding effects. Ru PEI luminescence is similar in the presence and absence of DNA. Ru PEI (7900 Da) and linear PEI (L-PEI; 22,000 Da) fully entrapped DNA (5.4 kb; pcDNA) at an N/P ratio of 2. LNCaP prostate cancer cells were transfected with a plasmid encoding for green fluorescent protein using Ru PEI and L-PEI vectors for comparison. For N/P = 48, the transfection efficiency for Ru PEI was approximately 50% relative to that of L-PEI.
通过对钌（II）三联吡啶中心的聚（2-乙基-2-恶唑啉）（Ru PEOX）进行酸水解合成了钌（II）三联吡啶中心的聚（乙烯亚胺）（Ru PEI），并对其发光、DNA包封及转染效率进行了评估。与Ru PEOX前体相比，Ru PEI样品的发射最大值发生红移，且在甲醇和水溶液中的发光寿命均较短。相对于[Ru(bpy)3]Cl2（bpy = 联吡啶），Ru PEOX和Ru PEI发光的氧猝灭较慢，这归因于聚合物屏蔽效应。在有和没有DNA的情况下，Ru PEI的发光相似。Ru PEI（7900 Da）和线性PEI（L-PEI；22,000 Da）在N/P比为2时完全包封了DNA（5.4 kb；pcDNA）。使用Ru PEI和L-PEI载体转染编码绿色荧光蛋白的质粒，对LNCaP前列腺癌细胞进行比较。对于N/P = 48，Ru PEI的转染效率相对于L-PEI约为50%。
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