Shuto Tsuyoshi, Furuta Takashi, Cheung Judy, Gruenert Dieter C, Ohira Yuko, Shimasaki Shogo, Suico Mary Ann, Sato Keizo, Kai Hirofumi
Department of Molecular Medicine, Graduate School of Pharmaceutical Sciences, Kumamoto University, Kumamoto 862-0973, Japan.
Leuk Res. 2007 Dec;31(12):1721-8. doi: 10.1016/j.leukres.2007.06.011. Epub 2007 Jul 30.
Neutrophils express functional toll-like receptor2 (TLR2) and 4 (TLR4) that are crucial for the production of inflammatory cytokines. Here, we show that dimethylsulfoxide-induced neutrophil-like differentiation of promyelocytic HL-60 cells (dHL-60) results in cells that respond to TLR2 and TLR4 ligands similarly to primary neutrophils. Consistent with the increased responsiveness of the cells to TLR2 ligand, the TLR2 gene was strongly up-regulated in dHL-60 cells. On the other hand, increased surface expression of LPS receptor complex, TLR4/MD2/CD14, was observed without affecting TLR4 gene expression. Thus, the data demonstrate a higher responsiveness of dHL-60 cells to TLR2 and TLR4 ligands because of increased TLR2 and MD2/CD14 gene expression.
中性粒细胞表达功能性Toll样受体2（TLR2）和4（TLR4），这对于炎性细胞因子的产生至关重要。在此，我们表明，二甲基亚砜诱导早幼粒细胞HL-60细胞向中性粒细胞样分化（dHL-60），所产生的细胞对TLR2和TLR4配体的反应与原代中性粒细胞相似。与细胞对TLR2配体反应性增加一致，TLR2基因在dHL-60细胞中强烈上调。另一方面，观察到脂多糖受体复合物TLR4/MD2/CD14的表面表达增加，而不影响TLR4基因表达。因此，数据表明dHL-60细胞对TLR2和TLR4配体具有更高的反应性，这是由于TLR2和MD2/CD14基因表达增加所致。
