Gene Regulation Laboratory, School of Biotechnology, Jawaharlal Nehru University, New Delhi 110 067, India.
Evid Based Complement Alternat Med. 2012;2012:732404. doi: 10.1155/2012/732404. Epub 2012 May 13.
Momordica charantia (bitter gourd) has been used in the traditional system of medicine for the treatment of various diseases. Anticancer activity of M. charantia extracts has been demonstrated by numerous in vitro and in vivo studies. In the present study, we investigated the differentiation inducing potential of fractionated M. charantia seed extracts in human myeloid HL60 cells. We found that the HL60 cells treated with the fractionated seed extracts differentiated into granulocytic lineage as characterized by NBT staining, CD11b expression, and specific esterase activity. The differentiation inducing principle was found to be heat-stable, and organic in nature. The differentiation was accompanied by a downregulation of c-myc transcript, indicating the involvement of c-myc pathway, at least in part, in differentiation. Taken together these results indicate that fractionated extracts of M. charantia seeds possess differentiation inducing activity and therefore can be evaluated for their potential use in differentiation therapy for leukemia in combination with other inducers of differentiation.
苦瓜（Momordica charantia）在传统医学体系中被用于治疗各种疾病。苦瓜提取物的抗癌活性已被众多体外和体内研究证明。在本研究中，我们研究了苦瓜种子提取物的分级部分在人髓系 HL60 细胞中的诱导分化潜力。我们发现，用分级种子提取物处理的 HL60 细胞分化为粒细胞系，其特征是 NBT 染色、CD11b 表达和特异性酯酶活性。诱导分化的原理是热稳定的，并且具有有机性质。分化伴随着 c-myc 转录本的下调，表明 c-myc 途径的参与，至少部分参与了分化。这些结果表明，苦瓜种子的分级提取物具有诱导分化活性，因此可以与其他诱导分化剂一起评估其在白血病分化治疗中的潜在用途。
