Breiner Boris, Schlatterer Jörg C, Alabugin Igor V, Kovalenko Serguei V, Greenbaum Nancy L
Department of Chemistry and Biochemistry, Florida State University, Tallahassee, FL 32306-4390, USA.
Proc Natl Acad Sci U S A. 2007 Aug 7;104(32):13016-21. doi: 10.1073/pnas.0705701104. Epub 2007 Jul 30.
Simple lysine conjugates are capable of selective DNA damage at sites approximating a variety of naturally occurring DNA-damage patterns. This process transforms single-strand DNA cleavage into double-strand cleavage with a potential impact on gene and cancer therapy or on the design of DNA constructs that require disassembly at a specific location. This study constitutes an example of DNA damage site recognition by molecules that are two orders of magnitude smaller than DNA-processing enzymes and presents a strategy for site-selective cleavage of single-strand nucleotides, which is based on their annealing with two shorter counterstrands designed to recreate the above duplex damage site.
简单的赖氨酸缀合物能够在接近多种自然发生的DNA损伤模式的位点进行选择性DNA损伤。这个过程将单链DNA切割转化为双链切割,可能会对基因和癌症治疗产生影响,或者对需要在特定位置进行拆解的DNA构建体的设计产生影响。本研究是比DNA加工酶小两个数量级的分子识别DNA损伤位点的一个例子,并提出了一种单链核苷酸位点选择性切割的策略,该策略基于它们与两条较短的互补链退火,以重新形成上述双链损伤位点。