Smaoui H, Mallie J P, Cheignon M, Borot C, Schaeverbeke J
Laboratoire de Biologie Cellulaire, Université de Paris, France.
Nephron. 1991;59(4):626-31. doi: 10.1159/000186655.
Alterations of tubules and glomerules have been reported previously in kidneys of rat neonates after aminoglycosides were given to the mother during gestation. Here, we have studied the effects of gentamicin on the development of the glomerular basement membrane (GBM). Pregnant Wistar female rates were treated with gentamicin. Deliveries occurred normally. Using electron microscopy, we looked at the deepest glomerules of the kidneys of 1-day-old neonates: myeloid bodies were found in podocytes, and the GBM appeared thicker and denser than in controls. Anionic ferritin, injected intravenously crossed the GBM in prenatally gentamicin-exposed animals, but not in controls. Furthermore, urine electrophoresis showed the presence of proteins normally found only in the urine of fetuses 2 days before birth. We suggest then, that in utero exposure to gentamicin leads to a delay of renal maturation and that the GBM is altered in juxtamedullary nephrons while it is normally differentiated and functioning in controls. Thus exposure to drugs before birth could be harmful to the GBM.
先前有报道称，在孕期给母鼠使用氨基糖苷类药物后，新生大鼠的肾小管和肾小球会出现改变。在此，我们研究了庆大霉素对肾小球基底膜（GBM）发育的影响。对怀孕的雌性Wistar大鼠使用庆大霉素进行治疗。分娩正常进行。利用电子显微镜，我们观察了1日龄新生大鼠肾脏最深层的肾小球：足细胞中发现了髓样小体，并且与对照组相比，GBM显得更厚且更致密。静脉注射的阴离子铁蛋白在产前暴露于庆大霉素的动物中穿过了GBM，但在对照组中未穿过。此外，尿液电泳显示存在通常仅在出生前2天胎儿尿液中才有的蛋白质。因此，我们认为子宫内暴露于庆大霉素会导致肾脏成熟延迟，并且在近髓肾单位中GBM发生改变，而在对照组中它正常分化并发挥功能。因此，出生前接触药物可能对GBM有害。
