Huang Kai-hong, Liu Jian-hua, Li Xue-xian, Song Li-bing, Zeng Mu-sheng
Department of Gastroenterology, Second Affiliated Hospital of Sun Yat-sen University, Guangzhou 510120, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2007 Jul;27(7):973-5.
To investigate the association of B cell-specific MLV integration site-1 (Bmi-1) mRNA expression level with the differentiation, metastasis and prognosis of gastric carcinoma.
Tissue specimens were obtained from 42 patients undergoing surgery for gastric carcinomas. Reverse transcriptional PCR (RT-PCR) was performed for amplification of Bmi-1 mRNA from the 42 tumor tissues and matched adjacent normal tissues, and the differential Bmi-1 mRNA expression was analyzed for its association with the clinical manifestations of the patients.
Bmi-1 mRNA expression was detected in all the gastric carcinoma tissues and normal tissues adjacent to the carcinoma using fluorescence method, and in 29 cases, Bmi-1 mRNA expression was significantly higher in the tumor tissues than in the adjacent tissues. Expression of Bmi-1 mRNA was highly correlated with tumor size, lymph node metastasis and T classification (P<0.05), but not with the patients' gender, age, or tumor differentiation (P>0.05). The survival rate was much lower in patients positive for Bmi-1 mRNA expression than in those without Bmi-1 mRNA expression.
Bmi-1 mRNA expression is correlated to differentiation and metastasis of gastric carcinoma and may facilitate its prognostic evaluation. Bmi-1 may serve as a marker for assessing the progression of gastric carcinoma and provide assistance for clinical therapeutic decisions.
探讨B细胞特异性MLV整合位点-1（Bmi-1）mRNA表达水平与胃癌分化、转移及预后的关系。
收集42例行胃癌手术患者的组织标本。采用逆转录聚合酶链反应（RT-PCR）从42例肿瘤组织及其配对的癌旁正常组织中扩增Bmi-1 mRNA，并分析Bmi-1 mRNA差异表达与患者临床表现的关系。
采用荧光法在所有胃癌组织及其癌旁正常组织中均检测到Bmi-1 mRNA表达，其中29例肿瘤组织中Bmi-1 mRNA表达明显高于癌旁组织。Bmi-1 mRNA表达与肿瘤大小、淋巴结转移及T分期高度相关（P<0.05），但与患者性别、年龄及肿瘤分化无关（P>0.05）。Bmi-1 mRNA表达阳性患者的生存率远低于无Bmi-1 mRNA表达的患者。
Bmi-1 mRNA表达与胃癌的分化和转移相关，可能有助于其预后评估。Bmi-1可作为评估胃癌进展的标志物，为临床治疗决策提供帮助。
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