荧光假单胞菌SBW25中性标记菌株的构建与验证

Construction and validation of a neutrally-marked strain of Pseudomonas fluorescens SBW25.

作者信息

Zhang Xue-Xian, Rainey Paul B

机构信息

NZ Institute of Advanced Study and Institute of Molecular Biosciences, Massey University, Private Bag 102 904, North Shore Mail Centre, Auckland, New Zealand.

出版信息

J Microbiol Methods. 2007 Oct;71(1):78-81. doi: 10.1016/j.mimet.2007.07.001. Epub 2007 Jul 10.

DOI:10.1016/j.mimet.2007.07.001

PMID:17669526

Abstract

Neutrally marked bacterial strains are useful in many experimental evolution and molecular ecology studies to assess the relative fitness of a given strain. Here we describe the construction and validation of a neutral marker for the model organism Pseudomonas fluorescens SBW25. The marked strain, called SBW25-lacZ, was created by integrating a promoterless 'lacZ into the defective prophage locus of the SBW25 chromosome. Fitness assays conducted in various laboratory media and in planta revealed that the fitness levels of SBW25-lacZ were comparable with the wild-type ancestor.

摘要

中性标记的细菌菌株在许多实验进化和分子生态学研究中可用于评估特定菌株的相对适合度。在此，我们描述了模式生物荧光假单胞菌SBW25中性标记的构建与验证。标记菌株SBW25-lacZ是通过将无启动子的“lacZ”整合到SBW25染色体的缺陷原噬菌体位点而创建的。在各种实验室培养基和植物体内进行的适合度测定表明，SBW25-lacZ的适合度水平与野生型祖先相当。