Kumar A Praveen, Reddy P Raveendra, Reddy V Krishna
Department of Chemistry, Sri Krishnadevaraya University, Anantapur 515003, Andhra Pradesh, India.
J Autom Methods Manag Chem. 2007;2007:48768. doi: 10.1155/2007/48768.
A simple and new simultaneous fourth derivative spectrophotometric method is proposed for the analysis of a two-component system containing cobalt(II) and nickel(II) without separation using 2-hydroxy-3-methoxy benzaldehyde thiosemicarbazone (HMBATSC) as a chromophoric reagent. The reagent reacts with cobalt(II) and nickel(II) at pH 6.0, forming soluble brown and yellow colored species, respectively. Cobalt(II) and nickel(II) present in themixture are simultaneously determined without solving the simultaneous equations bymeasuring the fourth derivative amplitudes at 468.5 nm and 474.5 nm, respectively. The derivative amplitudes obey Beer's law at 468.5 nm and 474.5 nm for Co(II) and Ni(II) in the range 0.059-3.299 mug mL(-1) and 0.058-3.285 mug mL(-1) respectively. A large number of foreign ions do not interfere in the present method. The present simultaneous method is used for the determination of micro amounts of cobalt in biological samples, nickel in plant samples, and in some alloy steels and soil sample.
提出了一种简单新颖的同时测定钴（II）和镍（II）两组分体系的四阶导数分光光度法，该方法以2-羟基-3-甲氧基苯甲醛缩氨基硫脲（HMBATSC）为显色剂，无需分离即可进行分析。该试剂在pH 6.0时与钴（II）和镍（II）反应，分别形成可溶性棕色和黄色物种。通过分别测量468.5 nm和474.5 nm处的四阶导数振幅，无需解联立方程即可同时测定混合物中的钴（II）和镍（II）。在468.5 nm和474.5 nm处，钴（II）和镍（II）的导数振幅在0.059 - 3.299 μg mL⁻¹和0.058 - 3.285 μg mL⁻¹范围内符合比尔定律。大量外来离子不干扰本法。本法用于测定生物样品中的微量钴、植物样品中的镍以及一些合金钢和土壤样品中的镍。
