Lin Sung-Jan, Jee Shiou-Hwa, Dong Chen-Yuan
Institute of Biomedical Engineering, National Taiwan University, No. 1, Sec. 1, Jen-Ai Road, Taipei 100, Taiwan.
Eur J Dermatol. 2007 Sep-Oct;17(5):361-6. doi: 10.1684/ejd.2007.0232. Epub 2007 Aug 2.
In recent years, the non-linear optical imaging technique of multiphoton microscopy has gained significant popularity in biomedical imaging. Since optical imaging can provide detailed morphological information of biological structures, multiphoton microscopy holds great promise as a potential clinical diagnostic tool of dermatological conditions. In this review, we will begin by discussing the basic principles of multiphoton microscopy, including the process of fluorescence and second harmonic generation. In addition, we will present the dermatological applications of multiphoton imaging, including the diagnosis of basal cell carcinoma and the evaluation of skin photoaging. We also describe applications of this technique to transcutaneous drug delivery, melanoma imaging, skin diseases associated with extracellular matrix alterations and cutaneous microvascular observation. Finally, we will discuss the additional issues that need to be resolved before multiphoton imaging can become a major diagnostic tool in clinical dermatology.
近年来，多光子显微镜的非线性光学成像技术在生物医学成像领域颇受关注。由于光学成像能够提供生物结构的详细形态学信息，多光子显微镜作为一种潜在的皮肤病临床诊断工具具有很大的前景。在本综述中，我们将首先讨论多光子显微镜的基本原理，包括荧光和二次谐波产生的过程。此外，我们将介绍多光子成像在皮肤病学中的应用，包括基底细胞癌的诊断和皮肤光老化的评估。我们还将描述该技术在经皮给药、黑色素瘤成像、与细胞外基质改变相关的皮肤疾病以及皮肤微血管观察方面的应用。最后，我们将讨论在多光子成像成为临床皮肤科主要诊断工具之前需要解决的其他问题。
