Department of Dermatology and Allergology, University Hospital, Jena, Germany.
Skin Res Technol. 2010 May;16(2):131-6. doi: 10.1111/j.1600-0846.2009.00423.x.
In the last years, multiphoton laser tomography (MLT) has emerged as a promising tool for non-invasive diagnostics in dermatology and other medical specialties. The present work is dedicated to the question to what degree the measurement depth and the thickness of the epidermis influence the evaluation of dermal matrix composition and if recommendations for future measurement procedures can be given.
In a study group of 30 healthy volunteers aged 21-82 years multiphoton depth-resolved measurements of autofluorescence and second harmonics have been performed in order to evaluate the dermal matrix composition.
Characteristic intensity curves depending on the penetration depth were derived and differences between age groups were found.
With the present work we provide evidence for the accuracy of the measurement of dermal matrix composition by MLT and give detailed advice for the measurement procedure. Furthermore, we propose the use of depth-dependent emission intensity curves for monitoring of anti-aging treatment.
在过去的几年中，多光子激光断层扫描（MLT）已经成为皮肤科和其他医学专业领域非侵入性诊断的一种有前途的工具。本工作旨在探讨测量深度和表皮厚度对真皮基质成分评估的影响程度，以及是否可以给出未来测量程序的建议。
在一个年龄为 21-82 岁的 30 名健康志愿者的研究组中，进行了多光子深度分辨的自发荧光和二次谐波测量，以评估真皮基质成分。
得出了依赖于穿透深度的特征强度曲线，并发现了不同年龄组之间的差异。
通过本工作，我们为 MLT 测量真皮基质成分的准确性提供了证据，并为测量程序提供了详细的建议。此外，我们建议使用深度相关的发射强度曲线来监测抗衰老治疗。
