Huang Long, Zhang Ru-song, Wang Cai-fang, Zhang Shan-fei
Zheng Dai Qing Chun Bao Pharmacy Co. Ltd, Huangzhou, China.
Zhong Yao Cai. 2007 Apr;30(4):415-7.
To inverstigate the chemical constituents of Patrinia villosa.
Under the direction of tetrazolium salt (MTf) assay, the chemical constituents were isolated by silica gel column chromatography and identified by physicochemical properties and spectral analysis (MS, FAB-MS, 1H NMR, 13C NMR).
Five compounds were isolated from petroleum ether and ethyl acetate extract and identified as: ursolic acid(I), beta-sitosterol (II), patrinalloside(III), kaempferol-3-O-beta-D-glucopyranoside(IV), and kaempferol-3-O-beta-D-glucopyranoside-7-O-alpha-L-rhamnoside(V).
Compounds I and V were obtained from the plant of genus Patrinia for the first time. Ursolic acid showed powerful anticancer activity through tetrazolium salt(MTT) assay, and its IC50 was 15.0 microg/ml.
研究白花败酱的化学成分。
在四氮唑盐（MTT）法指导下，采用硅胶柱色谱法分离化学成分，并通过理化性质和光谱分析（质谱、快原子轰击质谱、1H核磁共振、13C核磁共振）进行鉴定。
从石油醚和乙酸乙酯提取物中分离出5种化合物，分别鉴定为：熊果酸（I）、β-谷甾醇（II）、败酱皂苷（III）、山柰酚-3-O-β-D-吡喃葡萄糖苷（IV）和山柰酚-3-O-β-D-吡喃葡萄糖苷-7-O-α-L-鼠李糖苷（V）。
化合物I和V首次从败酱属植物中获得。通过四氮唑盐（MTT）法检测，熊果酸显示出强大的抗癌活性，其半数抑制浓度（IC50）为15.0微克/毫升。
