Zhuang Wei, Abramavicius Darius, Voronine Dimitrii V, Mukamel Shaul
Department of Chemistry, University of California, Irvine, CA 92697, USA.
Proc Natl Acad Sci U S A. 2007 Sep 4;104(36):14233-6. doi: 10.1073/pnas.0700392104. Epub 2007 Aug 3.
We propose to use infrared coherent two-dimensional correlation spectroscopy (2DCS) to characterize the fibril structure of Ab42, the dominant composition of Ab deposit, which is crucial for investigating its toxicity and aggregation mechanism. By optimizing the pulse polarization configurations with a genetic algorithm combined with sensitivity analysis, we obtained signals with well resolved cross-peak features attributed to the couplings within and between different structural motifs. These signals may provide new constraints for refining of the currently available NMR structure. Two-dimensional correlation spectroscopy also can differentiate the turn structure of Ab42 and other Ab derivatives.
我们建议使用红外相干二维相关光谱(2DCS)来表征淀粉样蛋白β42(Ab42)的纤维结构,Ab42是Ab沉积物的主要成分,这对于研究其毒性和聚集机制至关重要。通过结合灵敏度分析的遗传算法优化脉冲极化配置,我们获得了具有良好分辨交叉峰特征的信号,这些特征归因于不同结构基序内和之间的耦合。这些信号可能为完善目前可用的核磁共振结构提供新的约束条件。二维相关光谱还可以区分Ab42和其他Ab衍生物的转角结构。