通过代谢工程改造以提高植物螯合肽产量和镉积累量的细菌。
Bacteria metabolically engineered for enhanced phytochelatin production and cadmium accumulation.
作者信息
Kang Seung Hyun, Singh Shailendra, Kim Jae-Young, Lee Wonkyu, Mulchandani Ashok, Chen Wilfred
机构信息
Department of Chemical and Environmental Engineering, University of California, Riverside, CA 92521, USA.
出版信息
Appl Environ Microbiol. 2007 Oct;73(19):6317-20. doi: 10.1128/AEM.01237-07. Epub 2007 Aug 3.
Abstract
Phytochelatins (PCs) with good binding affinities for a wide range of heavy metals were exploited to develop microbial sorbents for cadmium removal. PC synthase from Schizosaccharomyces pombe (SpPCS) was overexpressed in Escherichia coli, resulting in PC synthesis and 7.5-times-higher Cd accumulation. The coexpression of a variant gamma-glutamylcysteine synthetase desensitized to feedback inhibition (GshI) increased the supply of the PC precursor glutathione, resulting in further increases of 10- and 2-fold in PC production and Cd accumulation, respectively. A Cd transporter, MntA, was expressed with SpPCS and GshI to improve Cd uptake, resulting in a further 1.5-fold increase in Cd accumulation. The level of Cd accumulation in this recombinant E. coli strain (31.6 micromol/g [dry weight] of cells) was more than 25-fold higher than that in the control strain.
摘要
对多种重金属具有良好结合亲和力的植物螯合肽（PCs）被用于开发去除镉的微生物吸附剂。粟酒裂殖酵母（SpPCS）的PC合酶在大肠杆菌中过表达，导致PC合成以及镉积累量提高了7.5倍。对反馈抑制脱敏的变体γ-谷氨酰半胱氨酸合成酶（GshI）的共表达增加了PC前体谷胱甘肽的供应，分别导致PC产量和镉积累量进一步提高了10倍和2倍。一种镉转运蛋白MntA与SpPCS和GshI一起表达以改善镉的摄取，导致镉积累量进一步提高了1.5倍。这种重组大肠杆菌菌株中的镉积累水平（31.6微摩尔/克[干重]细胞）比对照菌株高25倍以上。
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