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本文引用的文献

1
Selective cadmium accumulation using recombinant Escherichia coli.利用重组大肠杆菌进行选择性镉积累。
J Biosci Bioeng. 2005 Feb;99(2):109-14. doi: 10.1263/jbb.99.109.
2
Overexpression of genes involved in phytochelatin biosynthesis in Escherichia coli: effects on growth, cadmium accumulation and thiol level.大肠杆菌中植物螯合素生物合成相关基因的过表达：对生长、镉积累和硫醇水平的影响。
Acta Biochim Pol. 2005;52(1):109-16.
3
Metabolic pathway engineering to enhance aerobic degradation of chlorinated ethenes and to reduce their toxicity by cloning a novel glutathione S-transferase, an evolved toluene o-monooxygenase, and gamma-glutamylcysteine synthetase.通过克隆一种新型谷胱甘肽S-转移酶、一种进化的甲苯邻单加氧酶和γ-谷氨酰半胱氨酸合成酶进行代谢途径工程，以增强氯代乙烯的好氧降解并降低其毒性。
Environ Microbiol. 2004 May;6(5):491-500. doi: 10.1111/j.1462-2920.2004.00586.x.
4
Metal bioremediation through growing cells.通过生长细胞进行金属生物修复。
Environ Int. 2004 Apr;30(2):261-78. doi: 10.1016/j.envint.2003.08.001.
5
Enhanced accumulation of Cd2+ by a Mesorhizobium sp. transformed with a gene from Arabidopsis thaliana coding for phytochelatin synthase.用来自拟南芥的编码植物螯合肽合酶的基因转化的中生根瘤菌对Cd2+的积累增强。
Appl Environ Microbiol. 2003 Mar;69(3):1791-6. doi: 10.1128/AEM.69.3.1791-1796.2003.
6
Enhanced toxic metal accumulation in engineered bacterial cells expressing Arabidopsis thaliana phytochelatin synthase.在表达拟南芥植物螯合肽合酶的工程细菌细胞中增强的有毒金属积累。
Appl Environ Microbiol. 2003 Jan;69(1):490-4. doi: 10.1128/AEM.69.1.490-494.2003.
7
The Composition of Metals Bound to Class III Metallothionein (Phytochelatin and Its Desglycyl Peptide) Induced by Various Metals in Root Cultures of Rubia tinctorum.茜草根培养物中多种金属诱导产生的与III类金属硫蛋白（植物螯合素及其去甘氨酰肽）结合的金属组成
Plant Physiol. 1996 Apr;110(4):1145-1150. doi: 10.1104/pp.110.4.1145.
8
Genetic engineering of Escherichia coli for enhanced uptake and bioaccumulation of mercury.通过基因工程改造大肠杆菌以增强汞的摄取和生物累积
Appl Environ Microbiol. 2001 Nov;67(11):5335-8. doi: 10.1128/AEM.67.11.5335-5338.2001.
9
Escherichia coli gamma-glutamylcysteine synthetase. Two active site metal ions affect substrate and inhibitor binding.大肠杆菌γ-谷氨酰半胱氨酸合成酶。两个活性位点金属离子影响底物和抑制剂的结合。
J Biol Chem. 2002 Jan 4;277(1):50-8. doi: 10.1074/jbc.M107961200. Epub 2001 Oct 23.
10
Metal-binding proteins and peptides in bioremediation and phytoremediation of heavy metals.金属结合蛋白和肽在重金属生物修复和植物修复中的作用
Trends Biotechnol. 2001 Feb;19(2):67-73. doi: 10.1016/s0167-7799(00)01534-1.

通过代谢工程改造以提高植物螯合肽产量和镉积累量的细菌。

Bacteria metabolically engineered for enhanced phytochelatin production and cadmium accumulation.

作者信息

Kang Seung Hyun, Singh Shailendra, Kim Jae-Young, Lee Wonkyu, Mulchandani Ashok, Chen Wilfred

机构信息

Department of Chemical and Environmental Engineering, University of California, Riverside, CA 92521, USA.

出版信息

Appl Environ Microbiol. 2007 Oct;73(19):6317-20. doi: 10.1128/AEM.01237-07. Epub 2007 Aug 3.

DOI:10.1128/AEM.01237-07
PMID:17675418
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2075016/
Abstract

Phytochelatins (PCs) with good binding affinities for a wide range of heavy metals were exploited to develop microbial sorbents for cadmium removal. PC synthase from Schizosaccharomyces pombe (SpPCS) was overexpressed in Escherichia coli, resulting in PC synthesis and 7.5-times-higher Cd accumulation. The coexpression of a variant gamma-glutamylcysteine synthetase desensitized to feedback inhibition (GshI) increased the supply of the PC precursor glutathione, resulting in further increases of 10- and 2-fold in PC production and Cd accumulation, respectively. A Cd transporter, MntA, was expressed with SpPCS and GshI to improve Cd uptake, resulting in a further 1.5-fold increase in Cd accumulation. The level of Cd accumulation in this recombinant E. coli strain (31.6 micromol/g [dry weight] of cells) was more than 25-fold higher than that in the control strain.

摘要

对多种重金属具有良好结合亲和力的植物螯合肽（PCs）被用于开发去除镉的微生物吸附剂。粟酒裂殖酵母（SpPCS）的PC合酶在大肠杆菌中过表达，导致PC合成以及镉积累量提高了7.5倍。对反馈抑制脱敏的变体γ-谷氨酰半胱氨酸合成酶（GshI）的共表达增加了PC前体谷胱甘肽的供应，分别导致PC产量和镉积累量进一步提高了10倍和2倍。一种镉转运蛋白MntA与SpPCS和GshI一起表达以改善镉的摄取，导致镉积累量进一步提高了1.5倍。这种重组大肠杆菌菌株中的镉积累水平（31.6微摩尔/克[干重]细胞）比对照菌株高25倍以上。