Sharman P W A, Morrice D R, Law A S, Burt D W, Hocking P M
Institute of Evolutionary Biology, University of Edinburgh, Ashworth Laboratories, The King's Buildings, Edinburgh, UK.
Cytogenet Genome Res. 2007;117(1-4):296-304. doi: 10.1159/000103191.
An F2 broiler-layer cross was phenotyped for 18 skeletal traits at 6, 7 and 9 weeks of age and genotyped with 120 microsatellite markers. Interval mapping identified 61 suggestive and significant QTL on 16 of the 25 linkage groups for 16 traits. Thirty-six additional QTL were identified when the assumption that QTL were fixed in the grandparent lines was relaxed. QTL with large effects on the lengths of the tarsometatarsus, tibia and femur, and the weights of the tibia and femur were identified on GGA4 between 217 and 249 cM. Six QTL for skeletal traits were identified that did not co-locate with genome wide significant QTL for body weight and two body weight QTL did not coincide with skeletal trait QTL. Significant evidence of imprinting was found in ten of the QTL and QTL x sex interactions were identified for 22 traits. Six alleles from the broiler line for weight- and size-related skeletal QTL were positive. Negative alleles for bone quality traits such as tibial dyschondroplasia, leg bowing and tibia twisting generally originated from the layer line suggesting that the allele inherited from the broiler is more protective than the allele originating from the layer.
对一个F2代肉用型与蛋用型杂交群体在6周龄、7周龄和9周龄时的18个骨骼性状进行了表型测定，并用120个微卫星标记进行了基因分型。区间作图在25个连锁群中的16个上，针对16个性状鉴定出61个暗示性和显著性QTL。当放宽QTL在祖代品系中固定的假设时，又鉴定出36个额外的QTL。在GGA4上217至249 cM之间，鉴定出对跗跖骨、胫骨和股骨长度以及胫骨和股骨重量有较大影响的QTL。鉴定出6个骨骼性状的QTL，它们与全基因组显著的体重QTL不共定位，并且有2个体重QTL与骨骼性状QTL不一致。在10个QTL中发现了显著的印记证据，并且针对22个性状鉴定出QTL×性别互作。来自肉用型品系的与体重和大小相关的骨骼QTL的6个等位基因是正向的。诸如胫骨软骨发育不良、腿弯曲和胫骨扭转等骨骼质量性状的负向等位基因通常源自蛋用型品系，这表明从肉用型品系遗传的等位基因比源自蛋用型品系的等位基因更具保护性。
