Higashiguchi Takashi, Hotta Tsukasa, Takifuji Katsunari, Yokoyama Shozo, Matsuda Kenji, Tominaga Toshiji, Oku Yoshimasa, Yamaue Hiroki
Second Department of Surgery, School of Medicine, Wakayama Medical University, Wakayama, 641-8510, Japan.
Dis Colon Rectum. 2007 Oct;50(10):1585-93. doi: 10.1007/s10350-007-9016-3. Epub 2007 Aug 4.
Matrix metalloproteinase-7, reversion-inducing cysteine-rich protein with Kazal motifs, chemokine receptor 7, and vascular endothelial growth factor-C have been correlated with tumor invasion and lymph node metastasis in patients with gastrointestinal cancer. Our study of patients with colorectal cancer quantified the messenger ribonucleic acid (RNA) expressions at the deepest site of tumor invasion (the invasive front) and the tumor's inner surface. We investigated whether messenger RNA expressions can predict lymph node metastasis in colorectal cancer tumors and then evaluated the clinical implications of these results on the endoscopic treatment of early colorectal cancer.
Sixty patients with colorectal cancer participated in the study. Levels of matrix metalloproteinase-7, reversion-inducing cysteine-rich protein with Kazal motifs, chemokine receptor 7, and vascular endothelial growth factor-C messenger RNA in both the invasive front and inner surface of colorectal cancer tumors were measured by using real-time quantitative reverse transcriptase-polymerase chain reaction.
The matrix metalloproteinase-7 values in the invasive front and inner surface were significantly higher in tumors with lymph node metastasis than in tumors without lymph node metastasis. The matrix metalloproteinase-7 expression levels in the invasive front correlated with inner surface expression levels. Both a univariate and multiple logistic regression analysis showed the matrix metalloproteinase-7 expression levels in both the invasive front and inner surface of tumors to be significantly associated with lymph node metastasis.
Matrix metalloproteinase-7 messenger RNA in both the invasive front and inner surface of the tumor is a predictive factor associated with lymph node metastasis for patients with colorectal cancer. The results of this study might indicate further clinical applications of an endoscopic excision for cancer with submucosal invasion.
基质金属蛋白酶-7、含Kazal基序的富含半胱氨酸的逆转诱导蛋白、趋化因子受体7和血管内皮生长因子-C已被证实与胃肠道癌患者的肿瘤侵袭及淋巴结转移相关。我们对结直肠癌患者的研究，定量分析了肿瘤侵袭最深部位（侵袭前沿）及肿瘤内表面的信使核糖核酸（mRNA）表达情况。我们研究了mRNA表达是否可预测结直肠癌肿瘤的淋巴结转移，进而评估这些结果对早期结直肠癌内镜治疗的临床意义。
60例结直肠癌患者参与了本研究。采用实时定量逆转录-聚合酶链反应测定结直肠癌肿瘤侵袭前沿及内表面的基质金属蛋白酶-7、含Kazal基序的富含半胱氨酸的逆转诱导蛋白、趋化因子受体7和血管内皮生长因子-C信使RNA水平。
有淋巴结转移的肿瘤侵袭前沿及内表面的基质金属蛋白酶-7值显著高于无淋巴结转移的肿瘤。侵袭前沿的基质金属蛋白酶-7表达水平与内表面表达水平相关。单因素及多因素逻辑回归分析均显示，肿瘤侵袭前沿及内表面的基质金属蛋白酶-7表达水平均与淋巴结转移显著相关。
肿瘤侵袭前沿及内表面的基质金属蛋白酶-7信使RNA是结直肠癌患者淋巴结转移的预测因子。本研究结果可能提示内镜下切除黏膜下浸润癌的进一步临床应用。
