Zhu Kun, Qu Danni, Sakamoto Takanori, Fukasawa Ichio, Hayashi Masatoshi, Inaba Noriyuki
Department of Obstetrics and Gynecology, Dokkyo Medical University, 880 kita-Kobayashi, Mibu, Shimotsuga, Tochigi, Japan.
Arch Gynecol Obstet. 2008 Jan;277(1):15-9. doi: 10.1007/s00404-007-0423-4. Epub 2007 Aug 7.
Telomerase is composed primarily of catalytic subunit (hTERT) and RNA template (hTERC). Histone deacetylase (HDAC) inhibitors are known to modulate transcription and change the expression of hTERT and hTERC mRNA and telomerase activity in several types of cancer cells, but it is unclear if there is a similar effect in ovarian cancer cells.
The present study was designed to evaluate the effects of HDAC inhibitors on hTERT and hTERC mRNA expression in ovarian cancer cells. SK-V-3 cells were treated with the HDAC inhibitors, trichostatinA (TSA) and sodium butyrate (NaB); the expression of hTERC and hTERT mRNA and telomerase activity were evaluated by RT-PCR and TRAP assay, respectively.
In SK-OV-3 cells, TSA and NaB inhibited cell proliferation and induced apoptosis. The expression of hTERT and hTERT mRNA was not suppressed even after treatment with 1.0 microM TSA and 6 mM NaB, respectively. The telomerase activity was not changed by either TSA or NaB.
Histone deacetylase inhibitors inhibited cell proliferation and induced apoptosis, but had no effect on the expression of hTERC and hTERT mRNA and on telomerase activity.
端粒酶主要由催化亚基（hTERT）和RNA模板（hTERC）组成。已知组蛋白去乙酰化酶（HDAC）抑制剂可调节转录并改变几种癌细胞中hTERT和hTERC mRNA的表达以及端粒酶活性，但尚不清楚在卵巢癌细胞中是否有类似作用。
本研究旨在评估HDAC抑制剂对卵巢癌细胞中hTERT和hTERC mRNA表达的影响。用HDAC抑制剂曲古抑菌素A（TSA）和丁酸钠（NaB）处理SK-V-3细胞；分别通过逆转录聚合酶链反应（RT-PCR）和端粒重复序列扩增法（TRAP）检测hTERC和hTERT mRNA的表达以及端粒酶活性。
在SK-OV-3细胞中，TSA和NaB抑制细胞增殖并诱导凋亡。即使分别用1.0微摩尔/升TSA和6毫摩尔/升NaB处理后，hTERT和hTERT mRNA的表达也未受到抑制。TSA和NaB均未改变端粒酶活性。
组蛋白去乙酰化酶抑制剂抑制细胞增殖并诱导凋亡，但对hTERC和hTERT mRNA的表达以及端粒酶活性没有影响。
