小麦线条花叶病毒HC-Pro中保守半胱氨酸残基的替换消除了小麦曲叶螨对病毒的传播。
Substitution of conserved cysteine residues in wheat streak mosaic virus HC-Pro abolishes virus transmission by the wheat curl mite.
作者信息
Young B A, Hein G L, French R, Stenger D C
机构信息
United States Department of Agriculture-Agricultural Research Service, Department of Plant Pathology, University of Nebraska, Lincoln, NE, USA.
出版信息
Arch Virol. 2007;152(11):2107-11. doi: 10.1007/s00705-007-1034-x. Epub 2007 Aug 7.
Substitutions in the amino-proximal region of wheat streak mosaic virus (WSMV) HC-Pro were evaluated for effects on transmission by the wheat curl mite (Aceria tosichella Keifer). Alanine substitution at cysteine residues 16, 46 and 49 abolished vector transmission. Although alanine substitution at Cys(20) had no effect, substitution with arginine reduced vector transmission efficiency. Random substitutions at other positions (Lys(7) to Asn, Asn(19) to Ile, and Arg(45) to Lys) did not affect vector transmission. These results suggest that a zinc-finger-like motif (His(13)-X2-Cys(16)-X29-Cys(46)-X2-Cys(49)) in WSMV HC-Pro is essential for vector transmission.
对小麦线条花叶病毒（WSMV）HC-Pro氨基近端区域的替换进行了评估，以研究其对小麦曲叶螨（Aceria tosichella Keifer）传播的影响。半胱氨酸残基16、46和49处的丙氨酸替换消除了介体传播。虽然半胱氨酸（20）处的丙氨酸替换没有影响，但精氨酸替换降低了介体传播效率。其他位置（赖氨酸（7）替换为天冬酰胺、天冬酰胺（19）替换为异亮氨酸、精氨酸（45）替换为赖氨酸）的随机替换不影响介体传播。这些结果表明，WSMV HC-Pro中一个类似锌指的基序（组氨酸（13）-X2-半胱氨酸（16）-X29-半胱氨酸（46）-X2-半胱氨酸（49））对于介体传播至关重要。
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