Lazarevic I, Cupic M, Delic D, Svirtlih N S, Simonovic J, Jovanovic T
School of Medicine, Institute of Microbiology and Immunology, University of Belgrade, Belgrade, Serbia.
Arch Virol. 2007;152(11):2017-25. doi: 10.1007/s00705-007-1031-0. Epub 2007 Aug 6.
Hepatitis B virus (HBV) has been classified into eight genotypes (some of them further divided into two or more subgenotypes) and nine HBsAg subtypes, distinctly distributed geographically. The aim of this study was to gain insight into the distribution of HBV genotypes, subgenotypes and HBsAg subtypes among HBV chronically infected patients in Serbia, since there were no previously published data on this subject. Eighty-nine plasma samples that gave a positive result in a nested PCR were included for genotype identification. Genotyping was performed by direct sequencing of the part of the S/pol gene, and the HBsAg subtype was deduced from the HBsAg sequence. Two HBV genotypes, A and D, were encountered in Serbia, with genotype D (D - 82%, A - 18%) and subgenotype D3 (47.9%) being prevalent. Genotype D isolates had three assigned subtypes (ayw2, ayw3, ayw4), with ayw2 found to be the most prevalent (ayw2 - 53.4%, ayw3 - 43.8%, ayw4 - 1.4%). Genotype A isolates belonged to the A2 subgenotype and the HBsAg subtype adw2, as expected for samples from European population. The results correspond to country's geographical position, being in close proximity to the Mediterranean basin and on the main route between the Middle East and Central Europe.
乙型肝炎病毒（HBV）已被分为8个基因型（其中一些进一步分为两个或更多的亚基因型）和9个HBsAg亚型，在地理上分布明显。本研究的目的是深入了解塞尔维亚慢性HBV感染患者中HBV基因型、亚基因型和HBsAg亚型的分布情况，因为此前尚无关于该主题的公开数据。对89份巢式PCR检测呈阳性的血浆样本进行基因型鉴定。通过对S/pol基因部分进行直接测序来进行基因分型，并从HBsAg序列推导HBsAg亚型。在塞尔维亚发现了两种HBV基因型，A和D，其中基因型D（D - 82%，A - 18%）和亚基因型D3（47.9%）最为普遍。基因型D分离株有三种指定的亚型（ayw2、ayw3、ayw4），其中ayw2最为普遍（ayw2 - 53.4%，ayw3 - 43.8%，ayw4 - 1.4%）。基因型A分离株属于A2亚基因型和HBsAg亚型adw2，这与来自欧洲人群的样本预期一致。这些结果与该国的地理位置相符，该国靠近地中海盆地，且位于中东和中欧之间的主要路线上。
