Bartlam Mark, Xu Yuanyuan, Rao Zihe
College of Life Sciences, Nankai University, Tianjin, China.
J Struct Funct Genomics. 2007 Sep;8(2-3):85-97. doi: 10.1007/s10969-007-9024-5. Epub 2007 Aug 7.
A number of structural genomics/proteomics initiatives are focused on bacterial or viral pathogens. In this article, we will review the progress of structural proteomics initiatives targeting the SARS coronavirus (SARS-CoV), the etiological agent of the 2003 worldwide epidemic that culminated in approximately 8,000 cases and 800 deaths. The SARS-CoV genome encodes 28 proteins in three distinct classes, many of them with unknown function and sharing low similarity to other proteins. The structures of 16 SARS-CoV proteins or functional domains have been determined to date. Remarkably, eight of these 16 proteins or functional domains have novel folds, indicating the uniqueness of the coronavirus proteins. The results of SARS-CoV structural proteomics initiatives will have several profound biological impacts, including elucidation of the structure-function relationships of coronavirus proteins; identification of targets for the design of anti-viral compounds against SARS-CoV and other coronaviruses; and addition of new protein folds to the fold space, with further understanding of the structure-function relationships for several new protein families. We discuss the use of structural proteomics in response to emerging infectious diseases such as SARS-CoV and to increase preparedness against future emerging coronaviruses.
一些结构基因组学/蛋白质组学计划专注于细菌或病毒病原体。在本文中，我们将回顾针对严重急性呼吸综合征冠状病毒（SARS-CoV）的结构蛋白质组学计划的进展，SARS-CoV是2003年全球疫情的病原体，该疫情最终导致约8000例病例和800人死亡。SARS-CoV基因组编码三类共28种蛋白质，其中许多蛋白质功能未知，且与其他蛋白质的相似性较低。迄今为止，已确定了16种SARS-CoV蛋白质或功能域的结构。值得注意的是，这16种蛋白质或功能域中的8种具有新颖的折叠方式，表明冠状病毒蛋白质的独特性。SARS-CoV结构蛋白质组学计划的结果将产生几个深远的生物学影响，包括阐明冠状病毒蛋白质的结构-功能关系；确定针对SARS-CoV和其他冠状病毒设计抗病毒化合物的靶点；以及在折叠空间中增加新的蛋白质折叠方式，进一步了解几个新蛋白质家族的结构-功能关系。我们讨论了结构蛋白质组学在应对诸如SARS-CoV等新发传染病以及增强对未来新发冠状病毒的防范能力方面的应用。
