Sze Stephen Cho-Wing, Zhang Kalin Yan-Bo, Shaw Pang-Chui, But Paul Pui-Hay, Ng Tzi-Bun, Tong Yao
School of Chinese Medicine, University of Hong Kong, 10 Sassoon Road, Pokfulam, Hong Kong Special Administrative Region, People's Republic of China.
Biotechnol Appl Biochem. 2008 Feb;49(Pt 2):149-54. doi: 10.1042/BA20070059.
A DNA microarray was constructed for high-throughput identification of the plant resource of commercial FDSH [Fengdu Shihu (Dendrobium officinale)]. The 5 S rDNA (ribosomal DNA) intergenic spacer region in D. officinale, D. nobile, D. moniliforme, D. hercoglossum, D. williamsonii, D. capillipes, D. wilsonii and D. jenkinsii was amplified by a single primer pair and sequenced. The sequences showed polymorphism. They were incorporated on a glass slide and hybridized with fluorescently labelled 5 S sequences from commercial Shihu. The DNA microarray enabled the differentiation of D. officinale from the other species tested. FDSH could thus be distinguished from its adulterants. It is evident that DNA microarrays provide a high-throughput and reliable approach for the identification of plant resources, and the method presented here is useful for the authentication of FDSH.
构建了一种DNA微阵列，用于高通量鉴定市售枫斗石斛（铁皮石斛）的植物来源。用一对引物扩增并测序了铁皮石斛、金钗石斛、细茎石斛、曲茎石斛、霍山石斛、紫皮石斛、束花石斛和小黄花石斛的5S核糖体DNA（rDNA）基因间隔区。序列显示出多态性。将这些序列固定在载玻片上，并与来自市售石斛的荧光标记5S序列杂交。该DNA微阵列能够区分铁皮石斛与其他受试物种。因此，枫斗石斛可以与其掺假品区分开来。显然，DNA微阵列提供了一种高通量且可靠的植物资源鉴定方法，本文所提出的方法对枫斗石斛的鉴定很有用。
