[烟草花叶病毒外壳蛋白基因RNA干扰在转基因烟草植株中的表达赋予对烟草花叶病毒感染的免疫力]

[Expression of TMV coat protein gene RNAi in transgenic tobacco plants confer immunity to tobacco mosaic virus infection].

作者信息

Yan Pei-Qiang, Bai Xian-Quan, Wan Xiu-Qing, Guo Zhao-Kui, Li Li-Jie, Gong Hai-Ying, Chu Cheng-Cai

机构信息

Heilongjiang Tobacco Research Institute, Mudanjiang 157011, China.

出版信息

Yi Chuan. 2007 Aug;29(8):1018-22. doi: 10.1360/yc-007-1018.

DOI:10.1360/yc-007-1018

PMID:17681934

Abstract

RNAi technique has been proved as a powerful tool for plant breeding. In this paper, the coat protein of tobacco mosaic virus (TMV) was used for constructing the RNAi interference vector. The tobacco varieties K326 and Longjiang 911 were transformed via Agrobacterium tumefaciens-mediated transformation, and transgenic plants were generated. The expression analysis with real-time PCR indicated that TMV RNA had been degraded varied in different transgenic lines. Field assay revealed that 83% and 90 % transgenic plants showed immunity resistance to TMV in K326 and Longjiang 911 respectively.

摘要

RNA干扰技术已被证明是植物育种的有力工具。本文利用烟草花叶病毒（TMV）的外壳蛋白构建RNA干扰载体。通过根癌农杆菌介导的转化方法对烟草品种K326和龙江911进行转化，获得了转基因植株。实时荧光定量PCR表达分析表明，不同转基因株系中TMV RNA的降解情况有所不同。田间试验表明，K326和龙江911中分别有83%和90%的转基因植株对TMV表现出免疫抗性。