Zaĭdes V M, Selimova L M, Nikolaeva O G, Bukrinskaia A G
Vopr Virusol. 1976 Jan-Feb(1):44-50.
Inocluation of chick fibroblasts with Sendai virus results in a significant increase of protein synthesis. In cytoplasmic extracts of infected cells the content of polyribosomes increased and that of free (nontranslating) 80S ribosomes decreased, the "additional" polysomes of the infected cells being involved in protein synthesis. The portion of virus-specific protein synthesis in the infected cells was about 40% of the total protein synthesis. This means that with the total increase of protein synthesis 2-fold and (in some experiments) higher, the summary synthesis of cell proteins proper is not inhibited (possibly, it is slightly increased). Quite effective synthesis of virus-specific proteins for a comparatively long period after infection and ineffective maturation of virions eventually lead to a rather considerable accumulation of virus-specific proteins in the infected cells. The total content of protein in samples prepared 2 days after infection is approximately 1-1/2 as high as in uninfected samples.
用仙台病毒接种鸡胚成纤维细胞会导致蛋白质合成显著增加。在感染细胞的细胞质提取物中，多核糖体的含量增加，游离（非翻译）80S核糖体的含量减少，感染细胞的“额外”多核糖体参与蛋白质合成。感染细胞中病毒特异性蛋白质合成的比例约占总蛋白质合成的40%。这意味着，随着蛋白质合成总量增加2倍甚至更高（在某些实验中），细胞自身蛋白质的总合成并未受到抑制（可能略有增加）。感染后相当长一段时间内病毒特异性蛋白质的有效合成以及病毒粒子的无效成熟最终导致感染细胞中病毒特异性蛋白质的大量积累。感染后2天制备的样品中的蛋白质总含量约为未感染样品的1.5倍。
