在单光子或双光子激发下用荧光化学计量计追踪活细胞中的汞离子。

Tracking of mercury ions in living cells with a fluorescent chemodosimeter under single- or two-photon excitation.

作者信息

Lu Zhou-Jun, Wang Pei-Nan, Zhang Yu, Chen Ji-Yao, Zhen Shen, Leng Bing, Tian He

机构信息

State Key Lab for Advanced Photonic Materials and Devices, Department of Optical Science and Engineering, Fudan University, Shanghai 200433, China.

出版信息

Anal Chim Acta. 2007 Aug 6;597(2):306-12. doi: 10.1016/j.aca.2007.06.057. Epub 2007 Jul 7.

DOI:10.1016/j.aca.2007.06.057

PMID:17683744

Abstract

Tracking of Hg2+ in solutions as well as in living cells was conducted with a fluorescent chemodosimeter by measuring the spectral shift of its fluorescence under single- or two-photon excitation. The spectral hypsochromic shifts of this chemodosimeter when reacting with Hg2+ were found to be about 50 nm in acetonitrile/water solutions and 32 nm in Euglena gracilis 277 living cells. This chemodosimeter shows high sensitivity and selectivity, and is not influenced by the pH values. It can signal Hg2+ in solutions down to the ppb range under either single-photon excitation (SPE) at 405 nm or two-photon excitation (TPE) at 800 nm. However, with low cellular chemodosimeter concentrations, the SPE spectra were disturbed by the auto-fluorescence from the native fluorophore in the cell, while the TPE spectra were still of high quality since the two-photon absorption cross section of this chemodosimeter is much larger than that of the native fluorophores in the cell.

摘要

通过测量荧光化学传感器在单光子或双光子激发下荧光的光谱位移，对溶液以及活细胞中的Hg2+进行了追踪。发现该化学传感器与Hg2+反应时，在乙腈/水溶液中的光谱蓝移约为50 nm，在纤细裸藻277活细胞中的光谱蓝移为32 nm。这种化学传感器具有高灵敏度和选择性，不受pH值影响。在405 nm的单光子激发（SPE）或800 nm的双光子激发（TPE）下，它能够检测到溶液中低至ppb级别的Hg2+。然而，当细胞内化学传感器浓度较低时，SPE光谱会受到细胞内天然荧光团自发荧光的干扰，而TPE光谱质量仍然很高，因为该化学传感器的双光子吸收截面远大于细胞内天然荧光团的双光子吸收截面。