Department of Chemistry, Hue University, Hue City 84-234, Vietnam.
The University of Danang (University of Science and Education), Danang City 84-236, Vietnam.
Sensors (Basel). 2019 Jan 2;19(1):128. doi: 10.3390/s19010128.
A mercury sensor (-(rhodamine-6G)lactam-ethylenediamine-4-dimethylamino-cinnamaldehyde-RLED) based on the Hg-promoted hydrolysis reaction has been designed and developed with a combination of theoretical calculations and experimental investigations. The interaction between RLED and Hg goes through a fast-initial stage with formation of a 1:1 complex, followed by a slow hydrolysis process. The formation of durable intermediate complexes is due to quite a long hydrolysis reaction time. As a result, RLED can selectively detect Hg in the presence of other metal ions, with a detection limit of 0.08 μM for the colorimetric method, and of 0.008 μM with the fluorescent method. In addition, the RLED sensor can work in a solution with a small amount of organic solvent, with a wide pH range from 5 to 10. The time-dependent density functional theory has been used for investigations of the excitation and de-excitation processes in RLED, intermediate complexes, and reaction products, thereby clarifying the changes in the fluorescence intensity before and after the RLED interacts with Hg ions.
一种基于汞促进水解反应的汞传感器(-(罗丹明-6G)内酰胺-乙二胺-4-二甲基氨基肉桂醛-RLED)，通过理论计算和实验研究相结合的方式进行了设计和开发。RLED 与 Hg 的相互作用经历了一个快速初始阶段，形成了 1:1 配合物，然后是一个缓慢的水解过程。持久的中间配合物的形成是由于相当长的水解反应时间。因此，RLED 可以选择性地在存在其他金属离子的情况下检测 Hg，比色法的检测限为 0.08 μM，荧光法的检测限为 0.008 μM。此外，RLED 传感器可以在含有少量有机溶剂的溶液中工作，pH 值范围从 5 到 10。我们使用含时密度泛函理论研究了 RLED、中间配合物和反应产物中的激发和退激发过程，从而阐明了 RLED 与 Hg 离子相互作用前后荧光强度的变化。
