一种用于大量生产烟草疫霉菌游动孢子的简单体外“湿板”方法及影响游动孢子产生的因素。
A simple in-vitro 'wet-plate' method for mass production of Phytophthora nicotianae zoospores and factors influencing zoospore production.
作者信息
Ahonsi Monday O, Banko Thomas J, Hong Chuanxue
机构信息
Hampton Roads Agricultural Research and Extension Center, Virginia Polytechnic Institute and State University, 1444 Diamond Springs Road, Virginia Beach, VA 23455, USA.
出版信息
J Microbiol Methods. 2007 Sep;70(3):557-60. doi: 10.1016/j.mimet.2007.06.012. Epub 2007 Jun 30.
A simple in-vitro 'wet-plate' method for mass-producing Phytophthora nicotianae zoospores at > or =1.0 x 10(6) zoospores/ml is described. Temperature critically affected zoospore production; 22 degrees C was optimum, while 36 degrees C was completely inhibitory. Zoospores being the most important propagule of P. nicotianae, temperature of recycled irrigation water may be manipulated to reduce diseases in irrigated nursery crops.
本文描述了一种简单的体外“湿板”方法，用于大量生产烟草疫霉游动孢子，产量≥1.0×10⁶个游动孢子/毫升。温度对游动孢子的产生有至关重要的影响；22℃为最佳温度，而36℃则完全抑制游动孢子的产生。游动孢子是烟草疫霉最重要的繁殖体，因此可以通过控制循环灌溉水的温度来减少灌溉苗圃作物中的病害。
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