克氏锥虫KMP-11蛋白一个表位的免疫学及结构特征

Immunological and structural characterization of an epitope from the Trypanosoma cruzi KMP-11 protein.

作者信息

Díez H, Guzmán F, Alba M P, Cuéllar A, Thomas M C, López M C, Rosas F, Velasco V, González J M, Patarroyo M E, Puerta C J

机构信息

Laboratorio de Parasitología Molecular, Pontificia Universidad Javeriana, Bogotá, Colombia.

出版信息

Peptides. 2007 Aug;28(8):1520-6. doi: 10.1016/j.peptides.2007.06.017. Epub 2007 Jun 23.

DOI:10.1016/j.peptides.2007.06.017

PMID:17683828

Abstract

The K1 peptide is an HLA-A*0201-restricted cytotoxic epitope derived from the Trypanosoma cruzi KMP-11 protein, this being the etiological agent of Chagas' disease. This work describes the K1 peptide's secondary structure and its recognition by sera from chagasic patients. Circular dichroism and NMR spectroscopy analysis revealed that the K1 peptide adopts an alpha-helical conformation. Fifty-six percent of individuals had anti-K1 and 86% anti-KMP-11 antibodies by ELISA in the chronic Chagas' group and 28 and 68% in the indeterminate Chagas' group, respectively. By contrast, no reactivity was observed in sera from healthy individuals and tuberculosis patients. Antibody response subclass specificity to the K1 peptide was IgG1 and IgG3. Taken together these results support the idea that the K1 peptide acts as a B-cell-inducer epitope during Chagas' disease.

摘要

K1肽是一种源自克氏锥虫KMP - 11蛋白的HLA - A*0201限制性细胞毒性表位，克氏锥虫是恰加斯病的病原体。这项工作描述了K1肽的二级结构及其被恰加斯病患者血清识别的情况。圆二色性和核磁共振光谱分析表明，K1肽呈α - 螺旋构象。在慢性恰加斯病组中，通过酶联免疫吸附测定法，56%的个体有抗K1抗体，86%有抗KMP - 11抗体；在不确定型恰加斯病组中，分别为28%和68%。相比之下，在健康个体和结核病患者的血清中未观察到反应性。对K1肽的抗体反应亚类特异性为IgG1和IgG3。综合这些结果支持了K1肽在恰加斯病期间作为B细胞诱导表位起作用的观点。