Cárdenas Constanza, Guzmán Fanny, Carmona Marisela, Muñoz Cristian, Nilo Luis, Labra Alvaro, Marshall Sergio H
Núcleo Biotecnología Curauma, Pontificia Universidad Católica de Valparaíso, Valparaíso 2373223, Chile.
Laboratorio de Genética e Inmunología Molecular, Instituto de Biología, Pontificia Universidad Católica de Valparaíso, Valparaíso 2373223, Chile.
Pathogens. 2020 Jul 23;9(8):600. doi: 10.3390/pathogens9080600.
Viral infections in salmonids represent an ongoing challenge for the aquaculture industry. Two RNA viruses, the infectious pancreatic necrosis virus (IPNV) and the infectious salmon anemia virus (ISAV), have become a latent risk without healing therapies available for either. In this context, antiviral peptides emerge as effective and relatively safe therapeutic molecules. Based on in silico analysis of VP2 protein from IPNV and the RNA-dependent RNA polymerase from ISAV, a set of peptides was designed and were chemically synthesized to block selected key events in their corresponding infectivity processes. The peptides were tested in fish cell lines in vitro, and four were selected for decreasing the viral load: peptide GIM182 for IPNV, and peptides GIM535, GIM538 and GIM539 for ISAV. In vivo tests with the IPNV GIM 182 peptide were carried out using fish, showing a significant decrease of viral load, and proving the safety of the peptide for fish. The results indicate that the use of peptides as antiviral agents in disease control might be a viable alternative to explore in aquaculture.
鲑科鱼类的病毒感染对水产养殖业来说是一项持续的挑战。两种RNA病毒，传染性胰腺坏死病毒（IPNV）和传染性鲑鱼贫血病毒（ISAV），已成为潜在风险，目前尚无针对它们的治愈性疗法。在这种情况下，抗病毒肽成为有效且相对安全的治疗分子。基于对IPNV的VP2蛋白和ISAV的RNA依赖性RNA聚合酶的计算机分析，设计了一组肽并进行化学合成，以阻断其相应感染过程中的选定关键事件。这些肽在鱼类细胞系中进行了体外测试，其中四种被选来降低病毒载量：用于IPNV的肽GIM182，以及用于ISAV的肽GIM535、GIM538和GIM539。使用鱼类对IPNV GIM 182肽进行了体内测试，结果显示病毒载量显著降低，并证明了该肽对鱼类的安全性。结果表明，在疾病控制中使用肽作为抗病毒剂可能是水产养殖中值得探索的可行替代方案。
