分析克氏锥虫 KMP-11 和 HSP-70 重组抗原在恰加斯病患者体液免疫反应中的特征。
Characterising the KMP-11 and HSP-70 recombinant antigens' humoral immune response profile in chagasic patients.
机构信息
Laboratorio de Parasitología Molecular, Pontificia Universidad Javeriana, Cra, 7a No, 43-82, Ed, 50, Lab, 113, Bogotá, Colombia.
出版信息
BMC Infect Dis. 2009 Nov 25;9:186. doi: 10.1186/1471-2334-9-186.
BACKGROUND
Antigen specificity and IgG subclass could be significant in the natural history of Chagas' disease. The relationship between the different stages of human Chagas' disease and the profiles of total IgG and its subclasses were thus analysed here; they were directed against a crude T. cruzi extract and three recombinant antigens: the T. cruzi kinetoplastid membrane protein-11 (rKMP-11), an internal fragment of the T. cruzi HSP-70 protein 192-433, and the entire Trypanosoma rangeli HSP-70 protein.
METHODS
Seventeen Brazilian acute chagasic patients, 50 Colombian chronic chagasic patients (21 indeterminate and 29 cardiopathic patients) and 30 healthy individuals were included. Total IgG and its subtypes directed against the above-mentioned recombinant antigens were determined by ELISA tests.
RESULTS
The T. cruzi KMP-11 and T. rangeli HSP-70 recombinant proteins were able to distinguish both acute from chronic chagasic patients and infected people from healthy individuals. Specific antibodies to T. cruzi crude antigen in acute patients came from IgG3 and IgG4 subclasses whereas IgG1 and IgG3 were the prevalent isotypes in indeterminate and chronic chagasic patients. By contrast, the specific prominent antibodies in all disease stages against T. cruzi KMP-11 and T. rangeli HSP-70 recombinant antigens were the IgG1 subclass.
CONCLUSION
T. cruzi KMP-11 and the T. rangeli HSP-70 recombinant proteins may be explored together in the immunodiagnosis of Chagas' disease. Polarising the IgG1 subclass of the IgG response to T. cruzi KMP-11 and T. rangeli HSP-70 recombinant proteins could have important biological effects, taking into account that this is a complement fixing antibody.
背景
抗原特异性和 IgG 亚类在恰加斯病的自然史中可能具有重要意义。因此，分析了不同阶段的人类恰加斯病与总 IgG 及其亚类的特征之间的关系；它们针对的是一种粗制 T. cruzi 提取物和三种重组抗原：T. cruzi 动基体膜蛋白-11(rKMP-11)、T. cruzi HSP-70 蛋白 192-433 的内部片段和整个 Trypanosoma rangeli HSP-70 蛋白。
方法
纳入了 17 名巴西急性恰加斯病患者、50 名哥伦比亚慢性恰加斯病患者（21 名不确定型和 29 名心脏病患者）和 30 名健康个体。通过 ELISA 试验测定针对上述重组抗原的总 IgG 及其亚型。
结果
T. cruzi KMP-11 和 T. rangeli HSP-70 重组蛋白能够区分急性和慢性恰加斯病患者以及感染者与健康个体。急性患者针对 T. cruzi 粗抗原的特异性抗体来自 IgG3 和 IgG4 亚类，而 IgG1 和 IgG3 是不确定型和慢性恰加斯病患者中主要的同种型。相比之下，在所有疾病阶段，针对 T. cruzi KMP-11 和 T. rangeli HSP-70 重组抗原的特异性突出抗体都是 IgG1 亚类。
结论
T. cruzi KMP-11 和 T. rangeli HSP-70 重组蛋白可能一起用于恰加斯病的免疫诊断。考虑到这是一种补体结合抗体，将针对 T. cruzi KMP-11 和 T. rangeli HSP-70 重组蛋白的 IgG 反应的 IgG1 亚类极化可能具有重要的生物学效应。
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